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Series GSE54794 Query DataSets for GSE54794
Status Public on Mar 17, 2014
Title Rbfox2 controls autoregulation in RNA binding protein networks
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We generated a global analysis of Rbfox2 splicing regulation combined with a highly specific, single nucleotide-resolution Rbfox2 RNA binding map. We found that Rbfox2 regulates the splicing and expression of many previously unknown targets, and particularly a number of RNA binding proteins (RBPs), by modulating alternative splicing coupled-NMD. Based on our observations of RBP-Rbfox2 co-regulation with a polarity predicted by Rbfox2 binding, we propose a model whereby Rbfox2 tunes autoregulatory splicing events to control RBP expression levels and in turn alter their respective splicing networks.
Overall design iCLIP for epitope-tagged Rbfox2 and control untagged Rbfox2; RNAseq of control and Rbfox2 knockdown in mouse embryonic stem cells
Contributor(s) Jangi M, Sharp PA
Citation(s) 24637117
Submission date Feb 07, 2014
Last update date May 15, 2019
Contact name Mohini Jangi
Organization name MIT
Department Biology
Lab Sharp
Street address 500 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (10)
GSM1324104 UntagFOX2_iCLIP
GSM1324106 shLuc_r1_RNAseq
BioProject PRJNA237658
SRA SRP036863

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Supplementary file Size Download File type/resource
GSE54794_Fox2KD_cuffdiff.txt.gz 1.5 Mb (ftp)(http) TXT
GSE54794_RAW.tar 37.1 Mb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data are available on Series record

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