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| Status |
Public on Jul 22, 2003 |
| Title |
SAM PMN Parturition |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
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| Summary |
Bovine blood neutrophil RNA from 4 primiparous Holstein cows was collected on days -7, 0, 0.25, and 1 (relative to parturition on day 0). RNA was pooled across the four animals at the independent time points to obtain the 20 ug necessary for use in a loop design. RNA pools were separated into two 10 ug aliquots prior to cDNA synthesis and dye coupling in a loop design. Each sample was labeled with Cy3 and Cy5 and run in paired hybridizations as follows: GSM8506 compaired day 0.25 and day -7, GSM8507 compaired day 1 and day 0, GSM8508 compaired day -7 and day 1, GSM8509 compaired day 0 and 0.25. This pairing strategy was utilized to directly compare day -7 samples with days 0.25 and 1 samples because results from previous experiments suggested that the most pronounced differences in gene expression would occur between these days.
Keywords = microarray, cDNA, bovine, neutrophil, parturition
Keywords: time-course
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| Contributor(s) |
Madsen SA, Chang L, Hickey M, Rosa G, Coussens PM, Burton JL |
| Citation(s) |
14583595 |
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| Submission date |
Jul 18, 2003 |
| Last update date |
Feb 23, 2012 |
| Contact name |
Sally A Madsen |
| E-mail(s) |
madsens1@msu.edu
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| Phone |
517-353-9815
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| Organization name |
Michigan State University
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| Department |
Animal Science
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| Lab |
Immunogenetics Laboratory
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| Street address |
B212 Anthony Hall
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| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA85295 |
| Supplementary data files not provided |
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