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Series GSE54110 Query DataSets for GSE54110
Status Public on Jul 03, 2014
Title Genome-wide analysis of SUMOylation effects on androgen receptor (AR) binding in PC-3 cells (ChIP-seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Androgen receptor (AR) plays an important regulatory role during prostate cancer development. AR’s transcriptional activity is regulated by androgenic ligands, but also by post-translational modifications. To study the role of the AR SUMOylation in genuine chromatin environment, we compared androgen-regulated gene expression and AR chromatin occupancy in PC-3 prostate cancer and HEK293 cell lines stably expressing wild-type (wt) or SUMOylation site-mutated AR (AR-K386R,K520R). Our genome-wide gene expression analyses reveal that the SUMOylation modulates the AR function in a target gene and pathway selective manner. The transcripts that are differentially regulated by androgen and SUMOylation are linked to cellular movement, cell death, cellular proliferation, cellular development and cell cycle. In line with these data, SUMOylation mutant AR cells proliferate faster and are more sensitive to apoptosis. Moreover, ChIP-seq analyses show that the SUMOylation modulates the chromatin occupancy of AR on many loci in a fashion that parallels with their differential androgen-regulated expression. De novo motif analyses show that other transcription factor-binding motifs are differentially enriched at the wtAR- and the AR-K386R,K520R-preferred genomic binding positions. Taken together, our data indicate that SUMOylation does not simply repress the AR activity, but it regulates AR’s interaction with the chromatin and the receptor’s target gene selection.
Overall design Androgen receptor (AR) genomic binding was studied in wild-type AR (wtAR) or SUMOylation-deficient AR (AR-K2R) stably expressing cells PC-3 cells, in biological dublicates. Cells were treated 1h either with 10 nM R1881 or vehicle and inputs were used as controls.
Contributor(s) Sutinen P, Malinen M, Heikkinen S, Palvimo JJ
Citation(s) 24981513
Submission date Jan 15, 2014
Last update date May 15, 2019
Contact name Päivi Sutinen
Organization name University of Eastern Finland
Department School of Medicine
Lab Biomedicine
Street address Yliopistonranta 1 E
City Kuopio
ZIP/Postal code 70211
Country Finland
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (8)
GSM1308234 PC3_wtAR_INPUT
GSM1308235 PC3_wtAR_EtOH
GSM1308236 PC3_wtAR_R1881_rep1
This SubSeries is part of SuperSeries:
GSE54202 SUMOylation modulates the transcriptional activity of androgen receptor in a target gene and pathway selective manner.
BioProject PRJNA235194
SRA SRP035416

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Supplementary file Size Download File type/resource
GSE54110_PC3_AR-K2R_EtOH_peaks.bed.gz 480 b (ftp)(http) BED
GSE54110_PC3_AR-K2R_R1881_rep1+2_peaks.bed.gz 292.9 Kb (ftp)(http) BED
GSE54110_PC3_wtAR_ETOH_peaks.bed.gz 6.2 Kb (ftp)(http) BED
GSE54110_PC3_wtAR_R1881_rep1+2_peaks.bed.gz 245.4 Kb (ftp)(http) BED
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