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Series GSE53986 Query DataSets for GSE53986
Status Public on Mar 31, 2014
Title NRROS negatively regulates ROS in phagocytes during host defense and autoimmunity
Organism Mus musculus
Experiment type Expression profiling by array
Summary Production of reactive oxygen species (ROS) is one of the important antimicrobial mechanisms of phagocytic cells. Enhanced oxidative burst requires these cells to be primed with agents such as IFNg and LPS with a synergistic effect of these agents on the level of the burst. However, excessive ROS generation will lead to tissue damage and has been implicated in a variety of inflammatory and autoimmune disease. Therefore, this process needs to be tightly regulated. In order to understand the genes regulating this process, we will treat bone marrow derived macrophages with above mentioned priming agents and study the gene expression.
We used microarrays to determine the changes in gene expression that occur in bone marrow derived macrophages after treatment with IFNg, LPS, or a combination of IFNg and LPS
Overall design Four condition experiment; Biological replicates: four replicates per condition
Contributor(s) Noubade R, Wong K, Ota N, Rutz S, Eidenschenk C, Ding J, Valdez PA, Peng I, Sebrell A, Caplazi P, DeVoss J, Soriano RH, Modrusan Z, Hackney JA, Sai T, Ouyang W
Citation(s) 24739962
Submission date Jan 10, 2014
Last update date Feb 11, 2019
Contact name Jason A Hackney
Organization name Genentech, Inc.
Department Department of Bioinformatics and Computational Biology
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM1304836 BMDM, untreated, 1
GSM1304837 BMDM, untreated, 2
GSM1304838 BMDM, untreated, 3
BioProject PRJNA234284

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53986_RAW.tar 60.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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