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Status |
Public on May 01, 2015 |
Title |
Conserved nutrient sensor O-GlcNAc transferase is integral to the C. elegans pathogen-specific immune response |
Organism |
Caenorhabditis elegans |
Experiment type |
Expression profiling by array
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Summary |
Discriminating pathogenic bacteria from energy-harvesting commensals is key to host immunity. Using mutants defective in the enzymes of O-linked N-acetylglucosamine (O-GlcNAc) cycling, we examined the role of this nutrient-sensing pathway in the Caenorhabidits elegans innate immune response. Using whole genome transcriptional profiling, O-GlcNAc cycling mutants exhibited deregulation of unique stress- and immune-responsive genes as well as genes shared with the p38 MAPK/PMK-1 pathway. Moreover, genetic analysis showed that deletion of O-GlcNAc transferase (ogt-1) yielded animals hypersensitive to the human pathogen S. aureus but not to P. aeruginosa. Genetic interaction studies further revealed that nutrient-responsive OGT-1 acts through the conserved ß-catenin (BAR-1) pathway and in concert with p38 MAPK/PMK-1 to modulate the immune response to S. aureus. The participation of the nutrient sensor O-GlcNAc transferase in an immunity module conserved from C. elegans to humans reveals an unexplored nexus between nutrient availability and a pathogen-specific immune response.
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Overall design |
In C. elegans, three mutant strains(genotypes used: N2 (wild-type), ogt-1 (ok1474), oga-1 (ok1207), and pmk-1 (km25)) were treated with the human pathogen S. aureus (SA) or P. aeruginosa(PA) and OP50 (E. coli control) with three biological replications.
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Contributor(s) |
Bond MR, Ghosh SK, Wang P, Hanover JA |
Citation(s) |
25474640 |
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Submission date |
Dec 30, 2013 |
Last update date |
Jul 06, 2016 |
Contact name |
WeiPing Chen |
E-mail(s) |
weipingChen@niddk.nih.gov
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Phone |
301-496-0175
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Organization name |
NIDDK/NIH
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Department |
GCL
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Lab |
Genomics Core Lab
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Street address |
Bldg 8, Room 1A11, NIDDK/NIH
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City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
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Platforms (1) |
GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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Samples (36)
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Relations |
BioProject |
PRJNA232730 |