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Series GSE53732 Query DataSets for GSE53732
Status Public on May 01, 2015
Title Conserved nutrient sensor O-GlcNAc transferase is integral to the C. elegans pathogen-specific immune response
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Discriminating pathogenic bacteria from energy-harvesting commensals is key to host immunity. Using mutants defective in the enzymes of O-linked N-acetylglucosamine (O-GlcNAc) cycling, we examined the role of this nutrient-sensing pathway in the Caenorhabidits elegans innate immune response. Using whole genome transcriptional profiling, O-GlcNAc cycling mutants exhibited deregulation of unique stress- and immune-responsive genes as well as genes shared with the p38 MAPK/PMK-1 pathway. Moreover, genetic analysis showed that deletion of O-GlcNAc transferase (ogt-1) yielded animals hypersensitive to the human pathogen S. aureus but not to P. aeruginosa. Genetic interaction studies further revealed that nutrient-responsive OGT-1 acts through the conserved ß-catenin (BAR-1) pathway and in concert with p38 MAPK/PMK-1 to modulate the immune response to S. aureus. The participation of the nutrient sensor O-GlcNAc transferase in an immunity module conserved from C. elegans to humans reveals an unexplored nexus between nutrient availability and a pathogen-specific immune response.
Overall design In C. elegans, three mutant strains(genotypes used: N2 (wild-type), ogt-1 (ok1474), oga-1 (ok1207), and pmk-1 (km25)) were treated with the human pathogen S. aureus (SA) or P. aeruginosa(PA) and OP50 (E. coli control) with three biological replications.
Contributor(s) Bond MR, Ghosh SK, Wang P, Hanover JA
Citation(s) 25474640
Submission date Dec 30, 2013
Last update date Jul 06, 2016
Contact name WeiPing Chen
Phone 301-496-0175
Organization name NIDDK/NIH
Department GCL
Lab Genomics Core Lab
Street address Bldg 8, Room 1A11, NIDDK/NIH
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (36)
GSM1299481 1-km25-OP50
GSM1299482 2-km25-OP50
GSM1299483 3-km25-OP50
BioProject PRJNA232730

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Supplementary file Size Download File type/resource
GSE53732_RAW.tar 122.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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