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| Status |
Public on Dec 23, 2014 |
| Title |
A Salmonella-encoded microRNA-like RNA facilitates bacterial invasion and intracellular replication via suppressing host cell inducible nitric oxide synthase |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
As the leading cause of food-borne illness in the world, Salmonella have evolved a sophisticated machinery to alter host cell function to promote virulence and survival.In this study, we compare production of non-coding RNAs between Salmonella-infected cells and mock infection cells. Using Solexa deep sequencing, we detected a panel of 19-24nt Salmonella-derived non-coding RNA fragments with considerable large copy numbers in human colonic epithelial HT-29 cells following Salmonella infection.The fragment with the highest copy number, Sal-1, was further validated by both quantitative reverse transcription polymerase chain reaction (qRT-PCR) and northern blot. The generation of Sal-1 requires the infection of host cells by Salmonella, and the processing of the Sal-1 “primary” or “precursor” to the mature Sal-1 in Salmonella-infected cells is Dicer-independent but Argonaute 2 (Ago2)-dependent. Functionally, Sal-1 suppresses the expression of colonic epithelial cell endogenous inducible nitric oxide synthase (iNOS) via targeting its open reading frame and thus reduces the bacterial resistance of host cells.
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| Overall design |
Screening and identification of Salmonella-encoded microRNA-like RNA fragments
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| Contributor(s) |
Gu H, Zhang T, Zhang C, Zen K |
| Citation(s) |
28539638 |
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| Submission date |
Dec 23, 2013 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Zhen Zhou |
| E-mail(s) |
darknesszhou@gmail.com
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| Organization name |
Nanjing University
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| Department |
School of Lifesciences
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| Street address |
22th,Hankou Road
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210093 |
| Country |
China |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA232459 |
| SRA |
SRP034691 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53586_RAW.tar |
13.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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