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Series GSE53586 Query DataSets for GSE53586
Status Public on Dec 23, 2014
Title A Salmonella-encoded microRNA-like RNA facilitates bacterial invasion and intracellular replication via suppressing host cell inducible nitric oxide synthase
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary As the leading cause of food-borne illness in the world, Salmonella have evolved a sophisticated machinery to alter host cell function to promote virulence and survival.In this study, we compare production of non-coding RNAs between Salmonella-infected cells and mock infection cells. Using Solexa deep sequencing, we detected a panel of 19-24nt Salmonella-derived non-coding RNA fragments with considerable large copy numbers in human colonic epithelial HT-29 cells following Salmonella infection.The fragment with the highest copy number, Sal-1, was further validated by both quantitative reverse transcription polymerase chain reaction (qRT-PCR) and northern blot. The generation of Sal-1 requires the infection of host cells by Salmonella, and the processing of the Sal-1 “primary” or “precursor” to the mature Sal-1 in Salmonella-infected cells is Dicer-independent but Argonaute 2 (Ago2)-dependent. Functionally, Sal-1 suppresses the expression of colonic epithelial cell endogenous inducible nitric oxide synthase (iNOS) via targeting its open reading frame and thus reduces the bacterial resistance of host cells.
Overall design Screening and identification of Salmonella-encoded microRNA-like RNA fragments
Contributor(s) Gu H, Zhang T, Zhang C, Zen K
Citation(s) 28539638
Submission date Dec 23, 2013
Last update date Sep 25, 2019
Contact name Zhen Zhou
Organization name Nanjing University
Department School of Lifesciences
Street address 22th,Hankou Road
City Nanjing
State/province Jiangsu
ZIP/Postal code 210093
Country China
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (2)
GSM1296417 control
GSM1296418 SE infection
BioProject PRJNA232459
SRA SRP034691

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53586_RAW.tar 13.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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