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| Status |
Public on Dec 31, 2013 |
| Title |
Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts (Agilent 1-color) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Artificial epigenetic switches are of increasing demand owing to the critical role of the dynamic epigenome in orchestrating genome-wide transcriptional activation. Recently, we divulged that certain epigenetically active small molecules called SAHA-PIPs containing the cell permeable pyrrole-imidazole polyamides (PIPs) as DNA recognition module and a histone deacetylases inhibitor SAHA as functional module, is capable of triggering targeted transcriptional activation of pluripotency and germ cell genes in mouse and human fibroblasts, respectively. Through microarray studies and functional analysis, here we demonstrate the first ever example about the remarkable ability of 32 different SAHA-PIPs to trigger transcriptional activation of their own unique set of genes and noncoding RNAs. QRT-PCR studies validated that certain SAHA-PIPs could induce several therapeutically important genes including KSR2 and SEMA6A to suggest its potential use as reagents capable of targeted transcriptional activation and as probe(s) to identify the functional relevance of the uncharacterized genes.
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| Overall design |
Gene induction by each individual SAHA-PIP was measured after treating human dermal fibroblasts at 48 h time-point. Two libraries of SAHA-PIPs (SAHA-PIP 1-16 and 17-32) were investigated in different experiments and each experiment includes a vehicle (DMSO) treatment as a negative control.
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| Contributor(s) |
Pandian GN, Taniguchi J, Sugiyama H |
| Citation(s) |
24457603 |
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| Submission date |
Dec 13, 2013 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Junichi Taniguchi |
| E-mail(s) |
j.tani@kuchem.kyoto-u.ac.jp
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| Organization name |
Kyoto University
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| Department |
Department of Chemistry, Graduate School of Science
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| Lab |
Sugiyama Laboratry
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| Street address |
Kitashirakawa-Oiwakecho, Sakyo-Ku
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| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (35)
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| This SubSeries is part of SuperSeries: |
| GSE53319 |
Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts |
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| Relations |
| BioProject |
PRJNA231684 |
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