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Series GSE53230 Query DataSets for GSE53230
Status Public on Jan 08, 2014
Title Gene expression analysis in liver collected from mice administered non-toxic, toxic, or severely toxic LNA sequences
Organism Mus musculus
Experiment type Expression profiling by array
Summary Development of LNA gapmers, antisense oligonucleotides used for efficient inhibition of target RNA expression, is limited by non-target mediated hepatotoxicity issues. In the present study, we investigated hepatic transcription profiles of mice receiving non-toxic and toxic LNA gapmers after a single and repeat administration.
 
Overall design To understand the mechanism of LNA gapmer-induced heptotoxicity in mice, we investigated the transcription profiles of liver RNA isolated from mice receiving non-toxic sequence (NTS-1), toxic sequence (TS-2), or severely toxic sequence (HTS-3) of LNA gapmers at 25 mg/kg (dose volume of 10 mL/kg) at 8, 16, or 72 hrs after a single administration (by subcutaneous injection ) using microarray analysis. We also investigated the transcription profiles of liver RNA isolated from mice receiving non-toxic sequence (NTS-1) or toxic sequence (TS-2) of LNA gapmers at 25 mg/kg (dose volume of 10 mL/kg) at 2 weeks after repeated administration (by subcutaneous injection ) using microarray analysis.
 
Contributor(s) Kakiuchi-Kiyota S, Mathialagan N
Citation(s) 24336348
Submission date Dec 11, 2013
Last update date Feb 11, 2019
Contact name Petra Koza-Taylor
E-mail petra.h.koza-taylor@pfizer.com
Organization name Pfizer
Street address Eastern Point Road
City groton
State/province CT
ZIP/Postal code 06340
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (56)
GSM1288118 Vehicle DPBS, 8 hrs, biological rep 1
GSM1288119 Vehicle DPBS, 8 hrs, biological rep 2
GSM1288120 Vehicle DPBS, 8 hrs, biological rep 3
Relations
BioProject PRJNA231176

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53230_RAW.tar 211.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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