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Series GSE53172 Query DataSets for GSE53172
Status Public on Nov 13, 2014
Title TET1 is a tumour suppressor that inhibits colon cancer growth by derepressing inhibitors of the WNT pathway
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Ten eleven translocation (TET) enzymes catalyse the oxidative reactions of 5-methylcytosine (5mC) to promote the demethylation process. The reaction intermediate 5-hydroxymethylcytosine (5hmC) has been shown to be abundant in embryonic stem cells and tissues, but strongly depleted in human cancers. Genetic mutations of TET2 gene were associated with lleukemia, whereas TET1 downregulation has been shown to promote malignancy in breast cancer. Here, we report that TET1 is downregulated in colon tumours from the initial stage. TET1 silencing in primary epithelial colon cells increase their cellular proliferation while its re-­­expression in colon cancer cells inhibits their proliferation and the growth of tumour xenografts even at later stages. We found that TET1 binds and maintains hypomethylated the promoter of the DKK genes inhibitors of the WNT signalling to promote their expression. Downregulation of TET1 during colon cancer initiation leads to repression, by DNA methylation the promoters of the inhibitors of the WNT pathway resulting in a constitutive activation of the WNT pathway. Thus the DNA hydroxymethylation mediated by TET1 controlling the WNT signalling is a key player of tumour growth. These results provide new insights for understanding how tumours escape cellular controls
 
Overall design Transcriptome analysis of Caco-2 cell line expressing TET1 protein.
Web link http://epigenetics.hugef-research.org/data.php
 
Contributor(s) Neri F, Oliviero S
Citation(s) 25362856
Submission date Dec 10, 2013
Last update date Dec 28, 2018
Contact name Francesco Neri
E-mail francesco.neri@hugef-torino.org
Organization name HuGeF
Street address Via Nizza 52
City Torino
State/province Italy
ZIP/Postal code 10126
Country Italy
 
Platforms (1)
GPL15456 Illumina HiScanSQ (Homo sapiens)
Samples (2)
GSM1285923 RNA-Seq_Caco-2_control
GSM1285924 RNA-Seq_Caco-2_TET1
Relations
BioProject PRJNA231040
SRA SRP033646

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53172_RAW.tar 196.5 Mb (http)(custom) TAR (of BEDGRAPH)
Raw data are available in SRA
Processed data provided as supplementary file

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