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Series GSE53171 Query DataSets for GSE53171
Status Public on Jun 10, 2014
Title Differential actions of selective frankincense essential oil versus non-selective sandalwood essential oil induced bladder cancer cell cytotoxicity: A microarray and bioinformatics approach
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background: Frankincense (Ru Xiang) and sandalwood (Tan Xiang) are ingredients used in traditional Chinese medicine, and have been recognized as cancer preventive and therapeutic agents. Hydrodistillation of frankincense gum resins and sandalwood heartwood to prepare essential oils is a method to extract biologically active ingredients from these plant-derived products. This study was designed to differentiate frankincense (Boswellia carterii) and sandalwood (Santalum album) induced anti-proliferative and pro-apoptotic activities in cultured human bladder cancer cells. Methods: Frankincense and sandalwood essential oils-mediated cytotoxicity was studied in established human bladder cancer J82 cells and immortalized normal human bladder urothelial UROtsa cells using a colorimetric assay. Essential oils-activated gene expression and pathway activation in human bladder cancer J82 cells were identified using high density microarray and bioinformatics techniques.
Results: Human bladder cancer cells were more sensitive to immortalized normal bladder cells with suppressed viability following frankincense essential oil exposure. In contrast, both cancerous and normal bladder cells responded to sandalwood essential with similar levels of cytotoxicity. Based on microarray and bioinformatics analyses, genes responsible for suppressing biological processes and apoptosis were induced in J82 cells by both essential oils. Although both frankincense and sandalwood essential oils activated common ontologies and canonical pathways leading to suppressed J82 cell viability and apoptosis, each essential oil had unique property on these cells. For example, heat shock proteins and histone core were ongologies regulated by frankincense essential oil, whereas transcription regulation and G-protein couple receptor were ontologies unique to sandalwood essential oil treatment. In addition, NRF-2 mediated oxidative stress was implicated as the primary cause of frankincense essential oil-induced J82 cell death; in contrast, DNA damage and cell cycle arrest might be attributed to sandalwood essential oil-mediated cytotoxicity. Conclusion: Based on cell biology and comprehensive gene expression analysis, our results provide a preliminary, yet focused characterization of genetic responses to frankincense and sandalwood essential oils with respect to their proposed anti-neoplastic properties. Modern biomedical technologies are powerful tools to study biological responses following treatments with traditional Chinese medicine, which always consist of complex chemical constituents.
Overall design To differentiate mechanisms of frankincense and sandalwood essential oils induced cytotoxicty in bladder cancer J82 cells, time-dependent transcriptoms expression was performed in cultured cells following essential oils treatments
Contributor(s) Dozmorov MG, Yang Q, Wu W, Wren J, Suhail MM, Woolley CL, Youn DG, Fung K, Lin H
Citation(s) 25006348
Submission date Dec 10, 2013
Last update date Mar 20, 2017
Contact name Mikhail Dozmorov
Phone 804-827-2055
Organization name Virginia Commonwealth University
Department Biostatistics
Street address 830 E Main St
City Richmond
State/province VA
ZIP/Postal code 23298
Country USA
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (19)
GSM1285904 TSA-2 p13, no treatment
GSM1285905 TSA-2 p13, Frankincense 1:800x dilution, 0.5h treatment
GSM1285906 TSA-2 p13, Frankincense 1:800x dilution, 1h treatment
BioProject PRJNA231078

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Supplementary file Size Download File type/resource
GSE53171_RAW.tar 3.9 Mb (http)(custom) TAR
GSE53171_non-normalized.txt.gz 2.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available on Series record
Processed data included within Sample table

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