NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE5259 Query DataSets for GSE5259
Status Public on Aug 21, 2006
Title Induction of pluripotent-like cells derived from MEF by defined factors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Differentiated cells can be reprogrammed to an embryonic-like state by transfer of their nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about the factors that induce this reprogramming. Here we show that the combination of four factors, Oct3/4, Sox2, c-Myc, and Klf4, can generate pluripotent-like stem cells directly from mouse embryonic or adult fibroblast cultures. Unexpectedly, Nanog was dispensable in this process. These cells, which we designated iPS (induced pluriopotent-like stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent-like cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
Keywords: cell type comparison
 
Overall design Total RNAs were isolated from indicated cells and labeled with Cy3. Hybridization was performed once for each sample.
 
Contributor(s) Takahashi K, Kumazaki M
Citation(s) 16904174
Submission date Jul 07, 2006
Last update date Dec 06, 2012
Contact name Shinya Yamanaka
E-mail yamanaka@cira.kyoto-u.ac.jp
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platforms (1)
GPL2881 Agilent-013326 Mouse Oligo Microarray (V2) G4121B (Feature Number version)
Samples (9)
GSM119300 Fbx15-null ES (rep1)
GSM119301 Fbx15-null MEF (rep1)
GSM119322 FBX15-iPS MEF4-7 (rep1)
Relations
BioProject PRJNA96481

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5259_RAW.tar 35.2 Mb (http)(custom) TAR (of TXT)
GSE5259_ReadMe.txt 972 b (ftp)(http) TXT

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap