|
Status |
Public on Nov 21, 2013 |
Title |
Gene expression of HEK 293 cells transfected with Vector (PCMV), Aire, or MBD-VP16 |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
|
Summary |
HEK 293 cells were transiently transfected with plasmids expressing Vector only(PCMV), Aire, or MBD-VP16 with the goal of comparing the global gene expression profiles in the Aire and MBD-VP16 groups We used microarrays to detail the global gene expression profile.
|
|
|
Overall design |
HEK 293 cells were transiently transfected with plasmids expressing Vector only(PCMV), Aire, or MBD-VP16 and 72 hrs post transfection total RNA was collected for microarray analysis.
|
|
|
Contributor(s) |
Waterfield M, Anderson M |
Citation(s) |
24464130 |
Submission date |
Nov 20, 2013 |
Last update date |
Jul 26, 2018 |
Contact name |
Mark Stuart Anderson |
E-mail(s) |
manderson@diabetes.ucsf.edu
|
Organization name |
UCSF
|
Department |
Diabetes Center
|
Lab |
Anderson Lab
|
Street address |
513 Parnassus Avenue, HSW Room 1102, Box 0540
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94143 |
Country |
USA |
|
|
Platforms (1) |
GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
Samples (9)
|
GSM1269602 |
293 transfected with Aire replicate #1 |
GSM1269603 |
293 transfected with Aire replicate #2 |
GSM1269604 |
293 transfected with Aire replicate #3 |
GSM1269605 |
293 transfected with MBDVP16 replicate #1 |
GSM1269606 |
293 transfected with MBDVP16 replicate #2 |
GSM1269607 |
293 transfected with MBDVP16 replicate #3 |
|
This SubSeries is part of SuperSeries: |
GSE52548 |
Aire co-opts the repressive ATF7ip/MBD1 protein complex for the induction of immune tolerance |
|
Relations |
BioProject |
PRJNA229364 |