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Series GSE52469 Query DataSets for GSE52469
Status Public on Dec 31, 2013
Title Cyclin-dependent kinase 6 is a chromatin-bound cofactor for nuclear factor kappaB-dependent gene expression [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Given the intimate link between inflammation and dysregulated cell proliferation in cancer we investigated cytokine-triggered gene expression in different cell cycle stages. Transcriptome analysis revealed that G1 release through CDK6 and CDK4 primes and cooperates with the cytokine-driven gene response. CDK6 physically and functionally interacts with the NF-κB subunit p65 in the nucleus and is found at enhancers and promoters of many transcriptionally active NF-κB target genes. CDK6 recruitment to distinct chromatin regions of inflammatory genes was essential for proper loading of p65 to its cognate binding sites and for the function of p65 coactivators such as TRIP6. Furthermore, cytokine-inducible nuclear translocation and chromatin association of CDK6 depends on the kinase activity of TAK1 and p38. These results have widespread biological implications, as aberrant CDK6 expression or activation that is frequently observed in human tumors cooperates with NF-κB to shape the cytokine- and chemokine-repertoire in chronic inflammation and cancer.
 
Overall design 10 samples, five different antibodies for ChIP, no replicates, unstimulated cells as controls
 
Contributor(s) Jurida L, Kracht M, Bartkuhn M
Citation(s) 24389100
Submission date Nov 18, 2013
Last update date May 15, 2019
Contact name Marek Bartkuhn
E-mail(s) marek.bartkuhn@gen.bio.uni-giessen.de
Organization name Justus-Liebig-University Giessen
Department Institute for Genetics
Street address Heinrich-Buff-Ring 58
City Giessen
State/province Hessen
ZIP/Postal code 35392
Country Germany
 
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (10)
GSM1267402 CDK6 control KB
GSM1267403 CDK6 IL1 KB
GSM1267404 Pol II control KB
This SubSeries is part of SuperSeries:
GSE52470 Cyclin-dependent kinase 6 is a chromatin-bound cofactor for nuclear factor kappaB-dependent gene expression
Relations
BioProject PRJNA229063
SRA SRP033101

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52469_RAW.tar 1.5 Gb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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