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Series GSE52416 Query DataSets for GSE52416
Status Public on Jan 30, 2014
Title Effect of genetic Zfx deletion on Myc induced gene expression in bone marrrow myeloid progenitors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Acute myeloid leukemia (AML) and acute T-lymphoblastic leukemia (T-ALL) maintain the undifferentiated phenotype and proliferative capacity of their respective cells of origin, hematopoietic stem/progenitor cells and immature thymocytes. The mechanisms that maintain these progenitor-like characteristics are poorly understood. We report that the transcription factor Zfx is required for the development and propagation of experimental AML caused by MLL-AF9 fusion, and of T-ALL caused by Notch1 activation. In both leukemia types, Zfx activated progenitor-associated gene expression programs and prevented differentiation. Key Zfx target genes included mitochondrial enzymes Ptpmt1 and Idh2, whose overexpression partially rescued the propagation of Zfx-deficient AML. These studies identify a common mechanism that controls the cell-of-origin characteristics of acute leukemias derived from disparate lineages and transformation mechanisms.
 
Overall design Bone marrow progenitors (c-Kit +) from Zfx wt/y CreER mice and Zfx fl/y CreER mice were harvested after tamoxifen administration to induce Cre. The Zfx wt and Zfx ko progenitors were infected with Myc expressing and control retrovirus with GFP selectable marker and cultured for 48 hours in cytokine supplemented media. The GFP + myeloid progenitors were sorted and cultured for another 4 days prior to RNA extraction.
 
Contributor(s) Weisberg SP, Reizis BV
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Submission date Nov 15, 2013
Last update date Mar 04, 2019
Contact name Stuart Weisberg
E-mail(s) spw13@cumc.columbia.edu
Organization name Columbia University
Department Pathology and Cell Biology
Lab Weisberg
Street address 650 W 168th Street
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (12)
GSM1265776 Myc Zfx wt 1
GSM1265777 Myc Zfx ko 1
GSM1265778 GFP Zfx wt 1
This SubSeries is part of SuperSeries:
GSE43022 ZFX controls the propagation and cell-of-origin characteristics of acute leukemia
Relations
BioProject PRJNA228194

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52416_RAW.tar 53.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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