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Series GSE5239 Query DataSets for GSE5239
Status Public on Jul 07, 2006
Title Expression data from Escherichia coli
Platform organism Escherichia coli K-12
Sample organism Escherichia coli
Experiment type Expression profiling by array
Summary Transcription profiles in BL21, BL21/pOri1 and BL21/pOri2 were analysed using DNA microarray technology. BL21, BL21/pOri1 or BL21/pOri2 strains were cultured at chemostat status and harvested after the cultivation arrived steady status.
Keywords: Effects of plasmid DNA on Escherichia coli metabolism
 
Overall design BL21, BL21/pOri1 and BL21/pOri2 were cultured at the exponential growth status or at the same growth rate status, respectively. E. coli BL21, BL21/pOri1 and BL21/pOri2 RNAs were reverse-transcripted into cDNAs, The RNAs from the BL21, BL21/pOri1, BL21/pOri2 were labelled with biotin. The labelled cDNAs were mixed and then hybridized on the microarray slides. Experiments were repeated four times. Most of them have no significant difference comparing plasmid-carrying E. coli and plasmid-free E. coli
 
Contributor(s) Wang Z, Xiang L, Shao J, Wegrzyn G
Citation(s) 17112383
Submission date Jul 06, 2006
Last update date Mar 16, 2012
Contact name Dr Zhijun Wang, Ph.D
E-mail(s) zw37@cornell.edu, zjwang@shmu.edu.cn
Organization name Cornell University
Department Department of Population Medicine and Diagnostic Sciences
Street address Uptown road
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
 
Platforms (1)
GPL199 [Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array
Samples (24)
GSM118600 BL21(mu=0.46) (1)
GSM118601 BL21(mu=0.46) (2)
GSM118602 BL21(mu=0.46) (3)
Relations
BioProject PRJNA96451

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