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Series GSE52386 Query DataSets for GSE52386
Status Public on Dec 19, 2013
Title Rapid and Pervasive Changes in Genome-Wide Enhancer Usage During Mammalian Development
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Here we used epigenomic profiling for H3K27ac, a mark of active enhancers to examine the genome-wide in vivo utilization of enhancers in three different mouse tissues across seven developmental stages, ranging from mid-gestation through adulthood. The majority of the ~90,000 enhancers identified exhibited tightly temporally restricted activity windows and were associated with stage-specific biological functions and regulatory pathways in individual tissues. Comparative genomic analysis revealed that evolutionary conservation of enhancers decreases following mid-gestation across all tissues examined. The dynamic enhancer activities uncovered in this study illuminate rapid and pervasive temporal in vivo changes in enhancer usage underlying developmental processes and demonstrate the value of time-course chromatin profiling of relevant tissues across development.
Overall design Examination of H3K27ac in mouse forebrain, heart and liver tissues collected across developmental stages.
Contributor(s) Nord AS, Visel A, Pennacchio LA
Citation(s) 24360275
Submission date Nov 14, 2013
Last update date May 15, 2019
Contact name Alex S Nord
Organization name Lawrence Berkeley National Laboratory
Department Genomics Divsion
Street address 1 Cyclotron Road, Mail Stop 84-171
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (48)
GSM1264352 Forebrain E11.5 H3K27ac Rep1
GSM1264353 Forebrain E11.5 input Rep1
GSM1264354 Forebrain E11.5 H3K27ac Rep2
BioProject PRJNA227768
SRA SRP033009

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Supplementary file Size Download File type/resource
GSE52386_Dataset_Summary.xlsx.gz 46.9 Kb (ftp)(http) XLSX
GSE52386_RAW.tar 9.6 Gb (http)(custom) TAR (of BED, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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