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Series GSE52262 Query DataSets for GSE52262
Status Public on Nov 13, 2013
Title Expression data of CD24-CD44+ and ALDH+ cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary It has been suggested that breast cancers are driven and maintained by a cellular subpopulation with stem cell properties. These breast cancer stem cells (BCSCs) mediate metastasis and by virtue of their resistance to radiation and chemotherapy, contribute to relapse. Although several BCSC markers have been described, it is unclear whether these markers identify the same or independent BCSC populations.
Based on established breast cancer cell lines, as well as primary tumor xenografts, we show that BCSCs exist in distinct mesenchymal-like (epithelial-mesenchymal transition, EMT) and epithelial-like (mesenchymal-epithelial transition, MET) states characterized by expression of distinct markers, proliferative capacity and invasive characteristics. The gene expression profiles of mesenchymal-like and epithelial-like BCSCs are remarkably similar across the different molecular subtypes of breast cancer and resemble those of distinct basal and luminal stem cells found in the normal breast. We propose that the plasticity of BCSCs allowing them to transition between EMT- and MET-like states endows these cells with the capacity for tissue invasion, dissemination and growth at metastatic sites.
 
Overall design Breast cancer cell lines, primary xenografts and normal breast cells from patient were sorted using flow cytometry to select for cells that were CD24-,CD44+ and ALDH+. Gene expression profiles of CD24-CD44+ cells were compared with non-CD24-CD44+ cells. Gene expression profiles of ALDH+ cells were compared with ALDH- cells.
 
Contributor(s) Liu S, Washburn J, Johnson C
Citation(s) 24511467
Submission date Nov 12, 2013
Last update date Mar 25, 2019
Contact name yang CONG
E-mail(s) ycong@tmhs.org
Phone +1 713 441 8184
Organization name Houston Methodist Research Institution
Department Department of Systems Medicine and Bioengineering
Street address 6565 Fannin St.,
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (27)
GSM1262124 CD24-CD44+, ALDH- flow sorted cells from HCC1954 cell line
GSM1262125 CD24-CD44+, ALDH+ flow sorted cells from HCC1954 cell line
GSM1262126 non-CD24-CD44+, ALDH- flow sorted cells from HCC1954 cell line
Relations
BioProject PRJNA227310

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52262_RAW.tar 121.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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