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| Status |
Public on Mar 10, 2015 |
| Title |
m6A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
N6-methyladenosine (m6A) has been recently identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, but its features, regulatory mechanisms, and functions in cell reprogramming are largely unknown. Here, we report m6A modification profiles in the mRNA transcriptomes of four cell types with different degrees of pluripotency. Comparative analysis reveals several features of m6A, especially gene- and cell-type-specific m6A mRNA modifications. We also show that microRNAs (miRNAs) regulate m6A modification via a sequence pairing mechanism. Manipulation of miRNA expression or sequences alters m6A modification levels through modulating the binding of METTL3 methyltransferase to mRNAs containing miRNA targeting sites. Increased m6A abundance promotes the reprogramming of mouse embryonic fibroblasts (MEFs) to pluripotent stem cells; conversely, reduced m6A levels impede reprogramming. Our results therefore uncover a role for miRNAs in regulating m6A formation of mRNAs and provide a foundation for future functional studies of m6A modification in cell reprogramming.
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| |
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| Overall design |
m6A-seq in ESC, iPSC, NSC and sertoli cells.
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| |
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| Contributor(s) |
Chen T, Hao Y, Zhang Y, Wang X, Yang Y, Zhou Q |
| Citation(s) |
25683224 |
| |
| Submission date |
Nov 06, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Tong Chen |
| E-mail(s) |
chentong_biology@163.com
|
| Phone |
86-15210822685
|
| Organization name |
China Academy of Chinese Medical Sciences
|
| Department |
State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica
|
| Lab |
Luqi Huang's lab
|
| Street address |
No 16 Nan-xiao-jie Dong-zhi-men-nei
|
| City |
Beijing |
| ZIP/Postal code |
100700 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA226754 |
| SRA |
SRP032669 |
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