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| Status |
Public on Jul 02, 2007 |
| Title |
Effects of the Structural Maintenance of Chromosomes (SMC) protein on gene expression in Bacillus subtilis |
| Organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by array
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| Summary |
We monitored changes in Bacillus subtilis global gene expression in response to deletion or disruption of smc or ftsY.
Keywords: genetic modification
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| Overall design |
We extracted RNA, reverse transcribed, labeled, and co-hybridized samples to arrays containing PCR products amplified from >99% of B. subtilis genes. We performed at least three independent replicates of each experiment.
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| Contributor(s) |
Britton RA, Kuester-Schoeck E, Auchtung TA, Grossman AD |
| Citation(s) |
17416649 |
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| Submission date |
Jul 02, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Alan D. Grossman |
| E-mail(s) |
clee2@mit.edu, adg@mit.edu
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| Phone |
617-253-6702
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| Organization name |
MIT
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| Department |
Biology
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| Lab |
Grossman
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| Street address |
31 Ames Street, 68-530
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (2) |
| GPL3949 |
Bacillus subtilis WGD3 platform |
| GPL3950 |
Bacillus subtilis WGD11 platform |
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| Samples (19)
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| GSM118219 |
wild-type vs. smc deletion, replicate 1 |
| GSM118220 |
wild-type vs. smc deletion, replicate 2 |
| GSM118221 |
wild-type vs. smc deletion, replicate 3 |
| GSM118222 |
wild-type vs. smc deletion, replicate 4 |
| GSM118223 |
Wild-type, replicate 1 |
| GSM118224 |
Wild-type, replicate 2 |
| GSM118225 |
Wild-type, replicate 3 |
| GSM118226 |
ftsY disruption, replicate 1 |
| GSM118227 |
ftsY disruption, replicate 2 |
| GSM118228 |
ftsY disruption, replicate 3 |
| GSM118229 |
ftsY disruption, replicate 4 |
| GSM118230 |
smc disruption, replicate 1 |
| GSM118231 |
smc disruption, replicate 2 |
| GSM118232 |
smc disruption, replicate 3 |
| GSM118233 |
smc disruption, replicate 4 |
| GSM118234 |
smc deletion, replicate 1 |
| GSM118235 |
smc deletion, replicate 2 |
| GSM118236 |
smc deletion, replicate 3 |
| GSM118237 |
smc deletion, replicate 4 |
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| Relations |
| BioProject |
PRJNA96501 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5210_RAW.tar |
5.6 Mb |
(http)(custom) |
TAR (of GPR) |
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