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Series GSE52005 Query DataSets for GSE52005
Status Public on Mar 31, 2014
Title Trivalent Inactivated Influenza Vaccine (TIV) and Live Attenuated Influenza Vaccine (LAIV) Induce Different B cell and Transcriptional Responses in Children
Organism Homo sapiens
Experiment type Expression profiling by array
Summary LAIV and TIV are effective for prevention of influenza infection in children, but the mechanisms associated with protection are still not well defined. We analyzed the differences in B cell responses and transcriptional profiles. Compared to baseline, LAIV elicited a significant increase in naïve, memory and transitional B cells on day 30, while vaccination with TIV elicited an increase in number of plasmablasts on day 7. Antibody titers against the three vaccine strains (H1N1, H3N2 and B) were significantly higher in the TIV group and correlated with number of antibody-secreting cells. Regarding transcriptional profiles, both vaccines induced expression of interferon signaling, but at different time points, TIV on 1 day, and LAIV on day 7 day post-vaccination, the last only in children younger than 5 years old. Interferon-related genes over expressed in both vaccinated groups correlated with antibody titers of H3N2 vaccines strain. These results suggest that LAIV and TIV induced significant different B cell responses in vaccinated children. Early induction on interferon genes appears important for development of effective antibody responses.
Overall design Longitudinal study with time points at baseline (day 0), 24hr, day 7 and day 30. 136 total samples analyzed. This was a prospective cohort of previously healthy children 6 months to 14 years of age enrolled between October 2011 and February 2012. Thirty seven subjects were randomized to receive one dose of either LAIV (FluMist®, MedImmune) (n= 20) or TIV (Fluzone®, Sanofi Pasteur) (n= 17). Two exceptions were applied to the randomization: four children with ages between 6 months and 2 years old received TIV, since LAIV is not licensed on this age group, and four children with controlled asthma also received TIV following CDC’s recommendations. One child aged 6 months at the time of enrollment received two doses of the vaccine with one month interval apart, since it was his first influenza vaccination. Exclusion criteria included any medical comorbidities or chronic conditions (lung, kidney or heart disease), use of systemic steroids in the previous 2 weeks, and congenital or acquired immunodeficiencies. The 2011-2012 LAIV and TIV vaccines contained A/California/7/2009 (H1N1)-like, A/Perth/16/2009 (H3N2)-like and B/Brisbane/60/2008-like antigens, the same strains used on the 2010-2011 influenza vaccine. TIV recipients aged 6 to 35 months old received 0.25 mL intramuscularly and recipients aged 36 months and older received 0.5 mL. LAIV recipients received 0.2 mL by intranasal route, 0.1 mL per nostril. Blood samples were collected at four time points, on day zero prior to vaccination, and day 1, day 7 (range 6-8) and day 30 (range 27-33) post-vaccination.
Contributor(s) Cao RG, Obermoser G, Suarez NM, Lopez SC, Flano E, Mertz SE, Albrecht RA, Garcia-Sastre A, Mejias A, Blankenship D, Palucka K, Pascual V, Ramilo O
Citation(s) 24495909
Submission date Nov 01, 2013
Last update date Sep 15, 2020
Contact name Nicole Baldwin
Organization name BIIR
Street address 3434 Live Oak
City Dallas
ZIP/Postal code 75204
Country USA
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (140)
GSM1257128 NCH-VK-73B_TIV_24h
GSM1257129 NCH-VK-84C_LAIV_D7
GSM1257130 NHC-VK-60D_TIV_D30
BioProject PRJNA226092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52005_GSM1261854-GSM1261857_non-normalized.txt.gz 1.2 Mb (ftp)(http) TXT
GSE52005_RAW.tar 26.2 Mb (http)(custom) TAR
GSE52005_non-normalized.txt.gz 32.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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