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Series GSE51770 Query DataSets for GSE51770
Status Public on Jun 03, 2014
Title Interactions of ARF6 with PIF4, BZR1, and RGA integrate auxin signaling with environmental and other hormonal signals in Arabidopsis [ChIP-Seq]
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Auxin is a major plant hormone for both development and environmental adaptation. Auxin responses are context dependent and highly modulated by light, temperature, the circadian clock, brassinosteroid, and gibberellin, but the underlying mechanisms remain unclear. Here, we show that auxin signaling integrates with other signals through direct interactions of AUXIN RESPONSE FACTOR6 (ARF6) with PHYTOCHROME INTERACTING FACTOR4 (PIF4), the brassinosteroid-signaling transcription factor BZR1, and the gibberellin-signaling repressor RGA. ChIP-Seq and RNA-Seq experiments show that ARF6, PIF4, and BZR1 bind to largely overlapping targets in the genome and synergistically activate gene expression. In vitro and in vivo assays show that ARF6-promoter binding is enhanced by PIF4 and BZR1 but blocked by RGA. Furthermore, a tripartite HLH/bHLH module feedback regulates PIF activity and thus modulates auxin sensitivity according to additional developmental and environmental cues. Our results demonstrate a central growth-regulation transcriptional network that coordinates hormonal, environmental, and developmental control of cell elongation and plant growth.
Overall design Genome-wide identification of ARF6 DNA-binding sites in etiolated Arabidopsis seedlings.
Contributor(s) Oh E, Wang Z
Citation(s) 24867218
Submission date Oct 28, 2013
Last update date May 15, 2019
Contact name Eunkyoo Oh
Organization name Carnegie Institution
Department Plant Biology
Street address 260 Panama Street
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (2)
GSM1252254 ARF6 ChIP-Seq
GSM1252255 GFP ChIP-Seq
This SubSeries is part of SuperSeries:
GSE51773 Interactions of ARF6 with PIF4, BZR1, and RGA integrate auxin signaling with environmental and other hormonal signals in Arabidopsis
BioProject PRJNA225008
SRA SRP032273

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Supplementary file Size Download File type/resource
GSE51770_ARF6_peak.wig.gz 1.3 Mb (ftp)(http) WIG
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Raw data are available in SRA
Processed data are available on Series record

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