|
| Status |
Public on Oct 26, 2014 |
| Title |
University of Manitoba/MGCB2 C thermocellum RNA-seq Experiment |
| Organism |
Acetivibrio thermocellus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Determine overall gene expression profiles; RNA expression observed in stationary relative to exponential phase cells of strain Clostridum thermocellum (ATCC 27405) grown on cellobiose; Comparative RNA expression of 3 Clostridium thermocellu strains (DSM 1237, 2650, and 4150) grown to mid exponential phase on cellobiose; Verify whether observed differences in fermentation end-product ratios are reflected in differences in RNA expression profiles. RNA-seq data will be compared with the same experiments performed with proteomic experiments; Compare gene expression across relative strains.
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| |
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| Overall design |
Each test condition was provided as 2 biological replicates for primary strain and 1 replicate for comparing strain
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| |
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| Contributor(s) |
Sparling R, Levin DB, Rydzak T, Fristensky BW, Zhang X, Alvare GG, Björklund NK |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 26, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard Sparling |
| E-mail(s) |
Richard.Sparling@umanitoba.ca
|
| Organization name |
University of Manitoba
|
| Department |
Microbiology
|
| Street address |
418 Buller Building
|
| City |
Winnipeg |
| State/province |
MB |
| ZIP/Postal code |
R3T 0M2 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL17844 |
Illumina HiSeq 2000 (Clostridium thermocellum) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE51745 |
Comparison of gene expression profiles in three Clostridium thermocellum strains: DSM 1237, 2360 and 4150 |
|
| Relations |
| BioProject |
PRJNA224753 |
| SRA |
SRP032174 |
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