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Status |
Public on Oct 26, 2015 |
Title |
Expression of ATHB17 in Maize Increases Ear Weight at Silking |
Organism |
Zea mays |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Transformation of the Arabidopsis ATHB17 gene into maize results in the expression of a truncated protein (smaller by 113 amino acids) that functions as a dominant-negative regulator that can modify activity of endogenous maize HD-Zip II transcription factors. This RNASeq experiment indicates that the observed effects of ATHB17d113 on the maize ear inflorescence and ear transcriptome are very small. Expression of ATHB17delta113 protein in maize leads to changes in ear growth resulting in increased ear size at early reproductive stages and, potentially increased sink size.
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Overall design |
Two ATHB17delta113 expressing events (Event 1 and Event 2) were compared to control plants (herein referred to as WT) in the context of Monsanto Elite Maize hybrid line NN6306. Three bioreps of both Ear inflorescence and Ear tissues were sampled for the WT and each of the two transgenic events.
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Contributor(s) |
Khandelwal A, Morrell JA, Purushotham D, Huang MG, Caldo RA, Beazley K, Rice EA, Loida PJ |
Citation(s) |
24736658 |
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Submission date |
Oct 26, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Jim Morrell |
E-mail(s) |
jamorr1@monsanto.com
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Organization name |
Monsanto Co.
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Department |
Biotechnology
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Street address |
800 North Lindbergh Blvd
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City |
Saint Louis |
State/province |
Missouri |
ZIP/Postal code |
63167 |
Country |
USA |
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Platforms (1) |
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Samples (18)
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Relations |
BioProject |
PRJNA224746 |
SRA |
SRP032173 |