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Series GSE51742 Query DataSets for GSE51742
Status Public on Oct 26, 2015
Title Expression of ATHB17 in Maize Increases Ear Weight at Silking
Organism Zea mays
Experiment type Expression profiling by high throughput sequencing
Summary Transformation of the Arabidopsis ATHB17 gene into maize results in the expression of a truncated protein (smaller by 113 amino acids) that functions as a dominant-negative regulator that can modify activity of endogenous maize HD-Zip II transcription factors. This RNASeq experiment indicates that the observed effects of ATHB17d113 on the maize ear inflorescence and ear transcriptome are very small. Expression of ATHB17delta113 protein in maize leads to changes in ear growth resulting in increased ear size at early reproductive stages and, potentially increased sink size.
 
Overall design Two ATHB17delta113 expressing events (Event 1 and Event 2) were compared to control plants (herein referred to as WT) in the context of Monsanto Elite Maize hybrid line NN6306. Three bioreps of both Ear inflorescence and Ear tissues were sampled for the WT and each of the two transgenic events.
 
Contributor(s) Khandelwal A, Morrell JA, Purushotham D, Huang MG, Caldo RA, Beazley K, Rice EA, Loida PJ
Citation(s) 24736658
Submission date Oct 26, 2013
Last update date May 15, 2019
Contact name Jim Morrell
E-mail(s) jamorr1@monsanto.com
Organization name Monsanto Co.
Department Biotechnology
Street address 800 North Lindbergh Blvd
City Saint Louis
State/province Missouri
ZIP/Postal code 63167
Country USA
 
Platforms (1)
GPL15463 Illumina HiSeq 2000 (Zea mays)
Samples (18)
GSM1251894 WT_Ear_Rep1
GSM1251895 WT_Ear_Rep2
GSM1251896 WT_Ear_Rep3
Relations
BioProject PRJNA224746
SRA SRP032173

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51742_AtHB17_DESeq_SW_submit.csv.gz 2.8 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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