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Series GSE51708 Query DataSets for GSE51708
Status Public on Oct 26, 2013
Title Effects of CREB3L1 on gene expression
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The unfolded protein response (UPR) is activated in response to hypoxia-induced stress such as in the tumor microenvironment. This study examined the role of CREB3L1 (cAMP-responsive element-binding protein 3-like protein 1), a member of the UPR, in breast cancer development and metastasis. Initial experiments identified the loss of CREB3L1 expression in metastatic breast cancer cell lines compared to low- or non-metastatic cell lines. When metastatic cells were transfected with CREB3L1 they demonstrated reduced invasion and migration in vitro, as well as a significantly decreased ability to survive under non-adherent or hypoxic conditions. Interestingly, in an in vivo rat mammary tumor model, CREB3L1 expressing cells not only failed to form metastases compared to CREB3L1 null cells but regression of the primary tumors was seen in 70% of the animals as a result of impaired angiogenesis. Microarray and ChIP on Chip analyses identified changes in the expression of many genes involved in cancer development and metastasis, including a decrease in those involved in angiogenesis. These data suggest that CREB3L1 plays an important role in suppressing tumorgenesis and loss of expression is required for the development of a metastatic phenotype.
CREB3L1 is a member of the unfolded protein response family of proteins. CREB3L1 expression is lost from metastatic breast cancer cells. We wanted to determine the effctes CREB3L1 expression had on gene expression.
 
Overall design RNA was extracted from LN4D6 rats cells that were either untransfected or transfected with CREB3L1, changes in gene expression following transfection of CREB3l1 were then determined by hybridisation on Affymetrics microarrays.
 
Contributor(s) Anderson D, Mellor P
Citation(s) 24126059
Submission date Oct 25, 2013
Last update date Jul 31, 2017
Contact name Deborah Anderson
E-mail(s) deborah.anderson@saskcancer.ca
Phone 13069667038
Organization name University of Saskatchewan
Street address 107 Wiggins Road
City Saskatoon
State/province Saskatchewan
ZIP/Postal code S7N 5E5
Country Canada
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (6)
GSM1250914 PSM01_LN4D6 cells
GSM1250915 PSM02_LN4D6 cells
GSM1250916 PSM03_LN4D6 cells
This SubSeries is part of SuperSeries:
GSE51857 CREB3L1 is a metastasis suppressor that represses expression of genes regulating metastasis, invasion and angiogenesis
Relations
BioProject PRJNA224587

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51708_RAW.tar 17.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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