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Series GSE51681 Query DataSets for GSE51681
Status Public on May 07, 2015
Title Digital Gene Expression profiling of Schmidtea mediterranea stem cells.
Organism Schmidtea mediterranea
Experiment type Expression profiling by high throughput sequencing
Summary The freshwater planarian Schmidtea mediterranea is well known by its amazing regeneration capabilities thanks to the presence of adult stem cells, the neoblasts, the only proliferative cells and responsible for the differentiation in all the cell types of the organism. This study involves the creation of separated transcript libraries from both, isolated neoblasts and differentiated cells, as well as their posterior sequencing and quantification through Digital Gene Expression (DGE) for the characterization of the neoblast transcriptome.
Overall design Three DGE libraries were produced from FACS isolated cell populations X1 (proliferating stem cells, S/G2/M), X2 (a mix of proliferating stem cells and stem cell progeny, G0/G1) and Xin (differentiated cells, G0/G1). Cells were isolated from a pool of 32 regenerating animals two days after being cut off pre- and post-pharingeally to trigger regeneration and neoblast proliferation.
Contributor(s) Rodriguez-Esteban G, Saló E, Abril JF
Citation(s) 25952370
Submission date Oct 24, 2013
Last update date May 15, 2019
Contact name Gustavo Rodriguez-Esteban
Organization name Centro Nacional de Análisis Genómico
Department Development
Lab Single Cell Genomics
Street address C. Baldiri Reixac, 4; Barcelona Science Park
City Barcelona
State/province Catalonia
ZIP/Postal code 08028
Country Spain
Platforms (1)
GPL9327 Illumina Genome Analyzer (Schmidtea mediterranea)
Samples (3)
GSM1250441 Proliferating stem cells (S/G2/M)
GSM1250442 Proliferating stem cells and stem cell progeny (G0/G1)
GSM1250443 Differentiated cells (G0/G1)
BioProject PRJNA224556
SRA SRP031897

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51681_RAW.tar 660.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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