|
| Status |
Public on Sep 08, 2016 |
| Title |
Chromatin maps of human ASXL1, FOXK1 and O-GlcNAc |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Polycomb group (PcG) proteins are major determinants of gene silencing and epigenetic memory in higher eukaryotes. Acting in multimeric protein complexes, these factors control structure and function of chromatin. We used a robust affinity purification mass spectrometry (AP-MS) approach to systematically map the PcG protein interactome in a single human cell line. Importantly, we uncovered the topology map of the human PR-DUB de-ubiquitination complexes, which comprise the O-linked N-acetylglucosamine transferase OGT1 and a number of transcription factors. Here we provide genome-wide chromatin maps of identified PR-DUB1 subunits ASXL1, FOXK1 and the OGT1 catalyzed modification O-GlcNAc based on ChIP-seq.
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| |
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| Overall design |
Our data set comprises 3 ChIP-seq samples plus 2 input control samples using chromatin from Flp-In HEK293 T-REx cells cells which was immunoprecipitated using antibodies against ASXL1, FOXK1 and O-GlcNAc.
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| |
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| Contributor(s) |
Comoglio F, Beisel C |
| Citation(s) |
27705803 |
| Submission date |
Oct 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Christian Beisel |
| E-mail(s) |
christian.beisel@bsse.ethz.ch
|
| Phone |
+41 61 387 31 65
|
| Organization name |
ETH Zurich
|
| Department |
Biosystems Science and Engineering
|
| Lab |
Genomics Facility Basel
|
| Street address |
Mattenstrasse 26
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
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| Platforms (2) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (5)
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| Relations |
| BioProject |
PRJNA224307 |
| SRA |
SRP031894 |
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