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Series GSE51673 Query DataSets for GSE51673
Status Public on Sep 08, 2016
Title Chromatin maps of human ASXL1, FOXK1 and O-GlcNAc
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Polycomb group (PcG) proteins are major determinants of gene silencing and epigenetic memory in higher eukaryotes. Acting in multimeric protein complexes, these factors control structure and function of chromatin. We used a robust affinity purification mass spectrometry (AP-MS) approach to systematically map the PcG protein interactome in a single human cell line. Importantly, we uncovered the topology map of the human PR-DUB de-ubiquitination complexes, which comprise the O-linked N-acetylglucosamine transferase OGT1 and a number of transcription factors. Here we provide genome-wide chromatin maps of identified PR-DUB1 subunits ASXL1, FOXK1 and the OGT1 catalyzed modification O-GlcNAc based on ChIP-seq.
 
Overall design Our data set comprises 3 ChIP-seq samples plus 2 input control samples using chromatin from Flp-In HEK293 T-REx cells cells which was immunoprecipitated using antibodies against ASXL1, FOXK1 and O-GlcNAc.
 
Contributor(s) Comoglio F, Beisel C
Citation(s) 27705803
Submission date Oct 24, 2013
Last update date May 15, 2019
Contact name Christian Beisel
E-mail(s) christian.beisel@bsse.ethz.ch
Phone +41 61 387 31 65
Organization name ETH Zurich
Department Biosystems Science and Engineering
Lab Genomics Facility Basel
Street address Mattenstrasse 26
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (5)
GSM1250374 HEK293_ChIP_ASXL1
GSM1250375 HEK293_ChIP_FOXK1
GSM1250376 HEK293_ChIP_O-GlcNAc
Relations
BioProject PRJNA224307
SRA SRP031894

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Supplementary file Size Download File type/resource
GSE51673_RAW.tar 2.6 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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