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Series GSE51633 Query DataSets for GSE51633
Status Public on Jan 14, 2014
Title Brd4 and JMJD6-associated Anti-pause Enhancers in Regulation of Transcriptional Pause Release
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Distal enhancers characterized by H3K4me1 mark play critical roles in developmental and transcriptional programs. However, potential roles of specific distal regulatory elements in regulating RNA Polymerase II (Pol II) promoter-proximal pause release remain poorly investigated. Here we report that a unique cohort of jumonji C domain-containing protein 6 (JMJD6) and bromodomain-containing protein 4 (Brd4) co-bound distal enhancers, termed anti-pause enhancers (A-PEs), regulate promoter-proximal pause release of a large subset of transcription units via long-range interactions. Brd4-dependent JMJD6 recruitment on A-PEs mediates erasure of H4R3me2(s), which is directly read by 7SK snRNA, and decapping/demethylation of 7SK snRNA, ensuring the dismissal of the 7SKsnRNA/HEXIM inhibitory complex. The interactions of both JMJD6 and Brd4 with the P-TEFb complex permit its activation and pause release of regulated coding genes. The functions of JMJD6/ Brd4-associated dual histone and RNA demethylase activity on anti-pause enhancers have intriguing implications for these proteins in development, homeostasis and disease.
Overall design All Gro-seq(s) were designed to reveal the transcriptional targets of JMJD6 and Brd4, and assess the role of JMJD6 and Brd4 in Pol II promoter-proximal pause release.
All ChIP-seq(s) were designed to understand the unique features, associated molecular mechanisms and functions of the anti-pause enhancers (A-PEs) discovered in the current study.
Contributor(s) Liu W, Ma Q
Citation(s) 24360279
Submission date Oct 24, 2013
Last update date May 15, 2019
Contact name Qi Ma
Organization name UCSD
Street address 9500 Gilman Dr,
City San Diego
ZIP/Postal code 92093
Country USA
Platforms (2)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (40)
GSM1249869 Gro-seq-siCTL-re1-HEK293T
GSM1249870 Gro-seq-siJMJD6-1-re1-HEK293T
GSM1249871 Gro-seq-siJMJD6-2-re1-HEK293T
BioProject PRJNA224140
SRA SRP031885

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Supplementary file Size Download File type/resource
GSE51633_RAW.tar 7.0 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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