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Series GSE51483 Query DataSets for GSE51483
Status Public on May 19, 2014
Title Transcriptional Atlas of Cardiogenesis Maps Congenital Heart Disease Interactome
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mammalian heart development is built on highly conserved molecular mechanisms with polygenetic perturbations resulting in a spectrum of congenital heart diseases (CHD). However, the transcriptional landscape of cardiogenic ontogeny that regulates proper cardiogenesis remains largely based on candidate-gene approaches. Herein, we designed a time-course transcriptome analysis to investigate the genome-wide expression profile of innate murine cardiogenesis ranging from embryonic stem cells to adult cardiac structures. This comprehensive analysis generated temporal and spatial expression profiles, prioritized stage-specific gene functions, and mapped the dynamic transcriptome of cardiogenesis to curated pathways. Reconciling the bioinformatics of the congenital heart disease interactome, we deconstructed disease-centric regulatory networks encoded within this cardiogenic atlas to reveal stage-specific developmental disturbances clustered on epithelial-to-mesenchymal transition (EMT), BMP regulation, NF-AT signaling, TGFb-dependent induction, and Notch signaling. Therefore, this cardiogenic transcriptional landscape defines the time-dependent expression of cardiac ontogeny and prioritizes regulatory networks at the interface between health and disease.
Overall design To interrogate the temporal and spatial expression profiles across the entire genome during mammalian heart development, we designed a time-course microarray experiment using the mouse model at defined stages of cardiogenesis, starting with embryonic stem cells (ESC, R1 stem cell line), early embryonic developmental stages: E7.5 whole embryos, E8.5 heart tubes, left and right ventricle tissues at E9.5, E12.5, E14.5, E18.5 to 3 days after birth (D3) and adult heart (Figure 1A). At each time point, microarray experiments were performed on triplicate biological samples. Starting at E9.5, tissue samples from left ventricles (LV) and right ventricles (RV) were microdissected for RNA purification and microarray analysis to determine spatially differential gene expression between LV and RV during heart development.
Contributor(s) Li X, Martinez-Fernandez AJ, Terzic A, Nelson TJ
Citation(s) 24803680
Submission date Oct 21, 2013
Last update date Feb 11, 2019
Contact name XING LI
Organization name Mayo Clinic
Department Health Sciences Research
Street address 200 1st street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (45)
GSM1246305 Mouse R1 Stem Cells R1.1
GSM1246306 Mouse R1 Stem Cells R1.2
GSM1246307 Mouse R1 Stem Cells R1.3
BioProject PRJNA223290

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Supplementary file Size Download File type/resource
GSE51483_RAW.tar 163.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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