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Series GSE51434 Query DataSets for GSE51434
Status Public on Feb 13, 2017
Title Zebrafish gonadal transcriptome analysis in response to heat
Organism Danio rerio
Experiment type Expression profiling by array
Summary High temperature can induce masculinization in many fish species including zebrafish (Ospina-Alvarez & Piferrer PLoS One 3:e2837). Juvenile zebrafish exposed to high temperature during gonad differentiation period will result in male bias adult sex ratio. This experiment compared the zebrafish gonadal transcriptome between fishes that were exposed to high (36 oC) and control (28 oC) temperatures. Gene expression analysis using microarray were performed at juvenile (37 dpf) and adult (90 dpf) stages.
 
Overall design Juvenile zebrafish (18 dpf) were exposed to either high (36 oC) or control (28 oC) temperature treatment for 14 days. After the treatment both groups of fish were reared at 28 oC until 37 or 90 dpf. For analysis of the juvenile (37 dpf) 10 biological replicates (unknown sex) from each temperature treatment groups were compared. For the adult (90 dpf) female, 4 biological replicates from each temperature treatment groups were compared. For the adult (90 dpf) male, 10 biological replicates from each temperature treatment groups were compared.
 
Contributor(s) Liew WC, Orban L
Citation(s) 28115725
Submission date Oct 18, 2013
Last update date Feb 13, 2017
Contact name Woei Chang Liew
E-mail(s) wcliew@tll.org.sg
Organization name Temasek Life Sciences Laboratory
Lab Reproductive Genomics
Street address 1 Research Link, NUS
City Singapore
ZIP/Postal code 117604
Country Singapore
 
Platforms (1)
GPL17795 NimbleGen Danio rerio array [Zebrafish_Zv9-e70-2]
Samples (46)
GSM1245622 28_G01_M
GSM1245623 28_G02_Fe
GSM1245624 28_G03_Fe
Relations
BioProject PRJNA223170

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51434_RAW.tar 324.2 Mb (http)(custom) TAR (of PAIR)
Raw data provided as supplementary file
Processed data included within Sample table

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