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Series GSE51063 Query DataSets for GSE51063
Status Public on Jan 01, 2015
Title Redefinition of Human Androgen Responsive Elements [LNCaP_ChIP-exo]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The androgen receptor (AR) mediates the action of androgens by binding to androgen-responsive elements (AREs) and subsequently regulating target genes involved in prostate carcinogenesis. The precise locations, true nature, and functional roles of AREs in human prostate cancer are still unknown. Here we redefine AREs by motif-resolution AR chromatin immunoprecipitation-exonuclease (ChIP-exo) assay in human prostate cancer cells and tumors. Surprisingly, we find that, in addition to canonical full-length AREs and half-site-like AREs, a significant portion of the four redefined ARE categories comprises non-canonical full-length AREs. The redefined AREs in enhanced AR binding regions in prostate tumors versus paired non-malignant adjacent tissues regulate a prostate cancer-relevant gene network not only centered on AR, but more interestingly, on novel AR target genes mTOR, BIRC5 and BCL2L1 involved in prostate cancer cell growth and survival. The precise redefinition of AREs has important implications for understanding how AR contributes to prostate carcinogenesis.
Overall design To accurately define ARE in human genome and identify cancer related AR binding site, AR ChIP-exo is performed in LNCaP cells before/after androgen stimulation. Each experiment includes two replicates. Please see the PROTOCOLS section for detailed information.
Contributor(s) Wang Q, Chen Z
Citation(s) 25535248
Submission date Sep 20, 2013
Last update date May 15, 2019
Contact name Xun Lan
Phone 7738345917
Organization name Stanford University
Department Genetics
Lab Pritchard's Lab
Street address 318 Campus Dr. Room S240
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM1236921 Bicalutamide, replicate 1
GSM1236922 Bicalutamide, replicate 2
GSM1236925 Enzalutamide, replicate 1
This SubSeries is part of SuperSeries:
GSE43791 Redefinition of Human Androgen Responsive Elements
BioProject PRJNA220173
SRA SRP030128

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Supplementary file Size Download File type/resource
GSE51063_RAW.tar 11.6 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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