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| Status |
Public on Jan 15, 2014 |
| Title |
ChIP-seq of MDA-MB-134 cell line |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed ChIP seq experiment in MDA-MB-134 cell line in order to map the estrogen receptor alpha (ER) binding sites following the estrogen treatment in an ILC model. We have characterized the genome wide recruit of ER and scaned the binding sites for the presence of cofactor motifs. The binding peaks were also correlated to E2 regulated genes in this ILC model.
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| |
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| Overall design |
Four samples were subjected to high throughput sequencing: E-ER (estrogen treated followed by ER IP), E-IgG (estrogen treated followed by IgG), V-ER (EtOH treated followed by ER IP) and Input (MCF7 genomic DNA)
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| |
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| Contributor(s) |
Bahreini A, Sikora MJ |
| Citation(s) |
24425047 |
| |
| Submission date |
Sep 19, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Amir Bahreini |
| E-mail(s) |
bahreinia@upmc.edu
|
| Phone |
5712444045
|
| Organization name |
University of Pittsburgh
|
| Department |
Human Genetics
|
| Street address |
204 Craft Ave A430
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA219645 |
| SRA |
SRP030039 |
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