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| Status |
Public on Sep 17, 2016 |
| Title |
Genome-wide epigenetic analyses and Transcriptional analysis in NB4 cells expressing PML-RARalpha |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Illumina High-Throughput ChIP Sequencing profiling was performed using the H3K9K14ac antibody in NB4 cells treated with the compounds ATRA, MS-275,MC2392 (a hybrid molecule of ATRA with a 2-aminoanilide tail of the HDAC inhibitor MS-275) or solvent, DMSO. We find that MC2392 induces changes in H3 acetylation at a small subset of PML-RARα binding sites but also in regions not regulated by ATRA. Moreover, MC2392 alters expression of a number of stress-responsive and apoptotic genes.
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| Overall design |
Genome-wide ChIP-seq was performed in NB4 cells for histone3 acetylated in K9K14
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| Contributor(s) |
De Bellis F, Carafa V, Nebbioso A, Rotili D, Petraglia F, Conte M, Francoijs K, Ablain J, Valente S, Castellano R, Goubard A, Collette Y, Matarese F, Martens J, de Thé H, Mai A, Stunnenberg HG, Altucci L |
| Citation(s) |
24566867 |
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| Submission date |
Sep 17, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
H.G. Stunnenberg |
| E-mail(s) |
h.stunnenberg@ncmls.ru.nl
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| Phone |
+31-24-3610520
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| Organization name |
Radboud University
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| Department |
Department of Molecular Biology (274)
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| Street address |
Geert Grooteplein 28
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| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
USA |
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| Platforms (2) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA219427 |
| SRA |
SRP029992 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50958_RAW.tar |
1.5 Gb |
(http)(custom) |
TAR (of BED, WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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