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| Status |
Public on Jun 13, 2006 |
| Title |
Promotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1(E2F)does not require LIN-35(pRB) |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Microarray-based expression profiling of dissected gonads from efl-1, dpl-1 and lin-35 mutants reveals that EFL-1 and DPL-1 promote expression of an extensively overlapping set of target genes, consistent with the expectation that these two proteins function as a heterodimer. Regulatory regions upstream of many of these target genes have a canonical E2F binding site, suggesting that their regulation by EFL-1/DPL-1 is direct. Many EFL-1/DPL-1 responsive genes encode proteins required for oogenesis and early embryogenesis, rather than cell cycle components. By contrast, LIN-35 appears to function primarily as a repressor of gene expression in the germ line, and the genes that it acts on are for the most part distinct from those regulated by EFL-1 and/or DPL-1
Keywords: Mutant analysis of dpl-1, efl-1, and lin-35 in dissected C. elegans gonads
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| Overall design |
We isolated dissected gonads from dpl-1, efl-1 and lin-35 mutant adults and compared each to control dissected gonads. RNA was linearly amplified prior to labeling for all genotypes. Each comparison was done in triplicate (dpl-1 and lin-35) or in quadruplicate (efl-1) on independently grown and isolated animals.
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| Citation(s) |
16854972 |
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| Submission date |
Jun 13, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Valerie Reinke |
| E-mail(s) |
valerie.reinke@yale.edu
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| Phone |
203-785-5228
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| Organization name |
Yale University School of Medicine
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| Department |
Genetics
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| Lab |
Reinke lab
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| Street address |
333 Cedar St
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
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| Platforms (2) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA95509 |
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