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| Status |
Public on Aug 01, 2006 |
| Title |
Expression Profiling of MAP Kinase-Mediated Meiotic Progression in C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
A unique allele of MAP kinase, mpk-1(ga111), displays a reversible, temperature-sensitive, tissue-specific defect in progression through meiotic prophase I. We performed gene expression profiling on mpk-1(ga111) animals at multiple times after shift to permissive temperature to identify candidate downstream effectors of MPK-1 signaling in the germline. This analysis delineated a cohort of genes whose expression requires MPK-1 (MAP kinase) signaling in germ cells in the pachytene stage of meiosis I.
Keywords: Timecourse of C. elegans mpk-1 mutants
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| Overall design |
We analyzed six timepoints after MAP kinase mpk-1(ga111) mutants were shifted to a temperature permissive for meiotic progression. Each timepoint was sampled in triplicate. Control (mpk-1+) animals were sampled in triplicate at the beginning and end of the time series. All samples were compared to a common reference sample made of a mixture of both control samples.
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| Contributor(s) |
Leacock SW, Reinke V |
| Citation(s) |
17096596 |
| |
| Submission date |
Jun 12, 2006 |
| Last update date |
Sep 21, 2012 |
| Contact name |
Valerie Reinke |
| E-mail(s) |
valerie.reinke@yale.edu
|
| Phone |
203-785-5228
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| Organization name |
Yale University School of Medicine
|
| Department |
Genetics
|
| Lab |
Reinke lab
|
| Street address |
333 Cedar St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
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| Platforms (6)
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| GPL446 |
C_elegans_GHIstanfordprint_YMD2.gal.txt |
| GPL539 |
C_elegans_Aprint_YMD |
| GPL540 |
elegans_Bprint_YMD.gal.txt |
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| Samples (114)
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| Relations |
| BioProject |
PRJNA96705 |
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