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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 25, 2016 |
| Title |
PARVA promotes invasion through inducing phosphorylation of ILK signaling in lung cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Alpha-parvin (PARVA) is known to involve in the linkage of integrins, regulation of actin cytoskeleton dynamics, and cell survival. However, the role of PARVA in cancer progress is still unclear. Here, we identify PARVA as a potential oncogene from a lung cancer invasion cell line model by expression microarrays. Overexpression of PARVA enhances cell invasion, colony formation ability, and endothelial cell tube formation but knockdown of PARVA inhibits invasion and tube formation in vitro. PARVA also promotes tumorigenicity, angiogenesis, metastasis and mortality by in vivo tumorigenesis and metastasis mouse models. To explore the underlying mechanism, the PARVA-regulated signaling pathways were analyzed in PARVA-overexpressing cells compare with mock controls by expression microarrays.
We used microarrays to profile the global gene expression of PARVA-overexpressing cells compared with mock control cells and identified the pathways involved in PARVA-induced biofunctional alterations.
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| Overall design |
The stably pooled PARVA-overexpressing cells and mock control cells were selected with 1mg/ml G418 in CL1-0 cells, human lung adenocarcinoma cells. We compare the PARVA-expressing profiles with the mock controls to identify the possible mechanisms of PARVA-induced pathways in lung cancer cells.
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| Contributor(s) |
Huang A, Yu S, Chen JJ |
| Citation(s) |
25738875 |
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| Submission date |
Sep 06, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Sung-Liang Yu |
| E-mail(s) |
slyu@ntu.edu.tw
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| Phone |
886-2-23958341
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| Organization name |
National Taiwan University
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| Department |
Clinical Laboratory Sciences and Medical Biotechnology
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| Lab |
Microarray Core Facility
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| Street address |
Jen Ai Road Section1
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| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| GSM1225934 |
CL1-0 Mock cells, biological rep1 |
| GSM1225935 |
CL1-0 Mock cells, biological rep2 |
| GSM1225936 |
CL1-0 PARVA-overexpressing cells, biological rep1 |
| GSM1225937 |
CL1-0 PARVA-overexpressing cells, biological rep2 |
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| Relations |
| BioProject |
PRJNA218205 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50657_RAW.tar |
18.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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