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| Status |
Public on May 29, 2014 |
| Title |
Trophoblast giant cell underrepresentation (CGH) |
| Organism |
Mus musculus |
| Experiment type |
Genome variation profiling by genome tiling array
|
| Summary |
We characterized regions of underrepresentation that are specific to mouse polyploid trophoblast giant cells.
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| Overall design |
We performed array Comparative Genomics Hybridization (aCGH) to examine copy number variation (CNV) in mouse polyploid trophoblast giant cells (TGCs). We performed the following experiments in duplicates to examine CNV during various stages of in vivo and in vitro TGC development: e9.5 TGCs vs. embryonic controls, e11.5 TGCs vs. embryonic controls, e13.5 TGCs vs. embryonic controls, e16.5 TGCs vs. embryonic controls, as well as TGCs cultured 3, 5 and 7 days vs. 2N trophoblast stem cells. We also performed the following controls to show that underrepresentation is only found in polypoid trophoblast giant cells and not in either 2N placental cell types nor in other types of polyploid cells: 2N placenta disk vs. embryonic controls, 2N trophoblast stem cells vs. embryonic stem cells, and polyploid Megakaryocytes vs. embryonic controls. When possible, we performed arrays with the test and control samples of opposite sex (F-female, M-male), as an internal control for the array.
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| Contributor(s) |
Chuong EB, Hannibal RL, Baker JC |
| Citation(s) |
24785991 |
| |
| Submission date |
Sep 03, 2013 |
| Last update date |
Aug 28, 2014 |
| Contact name |
Roberta L Hannibal |
| E-mail(s) |
robertah@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10449 |
Agilent-027411 SurePrint G3 Mouse CGH Microarray 4x180K (Feature Number version) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE50585 |
Trophoblast giant cell underrepresentation |
|
| Relations |
| BioProject |
PRJNA217981 |
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