GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE50515 Query DataSets for GSE50515
Status Public on Sep 30, 2013
Title Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Drosophila melanogaster adult males perform an elaborate courtship ritual to entice females to mate. fruitless (fru), a gene that is one of the key regulators of male courtship behavior, encodes multiple male-specific isoforms (FruM). These isoforms vary in their carboxy-terminal zinc finger domains, which are predicted to facilitate DNA binding. By over-expressing individual FruM isoforms in fru-expressing neurons in either males or females and assaying the global transcriptional response by RNA-sequencing, we show that three FruM isoforms have different regulatory activities that depend on the sex of the fly. We identified several sets of genes regulated downstream of FruM isoforms.
Overall design RNA seqeuncing was performed on mRNA derived from adult male or female heads, for a total of 39 samples. These samples included two wild type genotypes (Berlin and Canton-S), two transheterozygous mutants for fru P1 (Df(3R)P14/Df(3R)fru4-40 and fruw12/ Df(3R)ChaM5), and 3 overexpressing genotypes (fru P1-Gal4: UAS-FruMA, UAS-FruMB, UAS-FruMC). There were at least 3 replicates from biological samples for all sex by genotype combinations.
Contributor(s) Dalton JE, Fear JM, Knott S, Baker B, McIntyre LM, Arbeitman MN
Citation(s) 24074028, 27172187
Submission date Aug 30, 2013
Last update date May 15, 2019
Contact name Michelle N Arbeitman
Organization name Florida State University
Department Medicine Biomedical Sciences
Street address 1115 W. Call Street
City Tallahassee
State/province Florida
ZIP/Postal code 32306
Country USA
Platforms (1)
GPL11203 Illumina Genome Analyzer IIx (Drosophila melanogaster)
Samples (39)
GSM1220611 AH_BerF_1
GSM1220612 AH_BerF_2
GSM1220613 AH_BerF_3
BioProject PRJNA220189
SRA SRP030129

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50515_RAW.tar 57.5 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap