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Series GSE50491 Query DataSets for GSE50491
Status Public on Feb 28, 2014
Title Regulation of the KEAP1/NRF2 oxidative stress response pathway by BRD4 in prostate and colorectal cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary To identify genes regulated by BRD4 and to provide insight into new mechanisms de-regulated by BRD4, such as the response to oxidative stress, we integrated BRD4-binding regions with BRD4 gene expression data. For this analysis we performed BRD4 chromatin immunoprecipitation experiments and BRD4 knock down experiments followed by RNA-Seq analyses. By integration of both gene lists we identified top candidate genes regulated by BRD4.
Overall design HEK cells have been investigated for genomewide BRD4 binding sites and expression changes after knock down of BRD4. Illumina sequencing was used to gather data of the type ChIP Seq and mRNA Seq.
Contributor(s) Hussong M, Boerno ST, Wunderlich A, Kerick M, Franz A, Timmermann B, Hirsch-Kauffmann M, Schweiger MR
Citation(s) 24763052
Submission date Aug 30, 2013
Last update date May 15, 2019
Contact name Michal Ruth Schweiger
Organization name Max Planck Institute For Molecular Genetics
Department Lehrach
Lab Schweiger
Street address Ihnestrasse 63-73
City Berlin
ZIP/Postal code 14195
Country Germany
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (8)
GSM1220017 RNA1_ctrl
GSM1220018 RNA2_KO_BRD4
GSM1220019 RNA3_ctrl
BioProject PRJNA217765
SRA SRP029365

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50491_RAW.tar 9.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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