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Series GSE50149 Query DataSets for GSE50149
Status Public on Nov 01, 2013
Title Quorum sensing in CD4+ T cells homeostasis : IL-2 coordinates the interplay between IL-2p and regulatory T cells.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Many species of bacteria use quorum sensing to sense the amounts of secreted metabolites and adapt their growth according to their population density. We asked whether similar mechanisms would operate in lymphocyte homeostasis. We investigated the regulation of the size of Interleukin-2-producing CD4+ T-cell (IL-2p) pool using different IL-2-reporter mice. We found that in the absence of either IL-2 or regulatory CD4+ T-cells (Treg) the number of IL-2p-cells increases. Administration of IL-2 decreases the number of cells of the IL-2p-cell subset and pertinently, abrogates their ability to produce IL-2 upon in vivo cognate stimulation, while increasing Treg-cell numbers. We propose that control of the IL-2p-cell numbers occurs via a quorum-sensing-like feedback loop where the produced IL-2 is sensed by both the activated CD4+ T-cell pool and by Treg-cells, which reciprocally regulate cells of the IL-2p-cell subset. In conclusion, IL-2 acts as a self-regulatory circuit integrating the homeostasis of activated and regulatory T cells as CD4+ T-cells restrain their growth by monitoring IL-2 levels thereby preventing uncontrolled responses and autoimmunity.
 
Overall design 2 populations of conventional CD4+ T cell are analysed. 5 replicates for each. GFP- is the control one.
 
Contributor(s) Amado IF, Berges J, JLuther R, Mailhé M, Garcia S, Bandeira A, Weaver C, Liston A, AFreitas A
Citation(s) 24249704
Submission date Aug 23, 2013
Last update date Feb 02, 2018
Contact name Antonio Angelo Freitas
E-mail(s) antonio.freitas@pasteur.fr
Organization name Institut Pasteur
Department Lymphocyte Population Biology Unit
Street address 25 Rue du Dr. Roux
City Paris
ZIP/Postal code 75015
Country France
 
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (10)
GSM1214748 M1_GFP+
GSM1214749 M2_GFP+
GSM1214750 M3_GFP+
Relations
BioProject PRJNA216373

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50149_RAW.tar 118.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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