|
| Status |
Public on Feb 13, 2014 |
| Title |
Tissue specific expression of chalcone synthase siRNAs |
| Organism |
Glycine max |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In a previous study, seed coat and cotyledon tissues of Williams, Richland and T157 soybean lines were investigated to show tissue specificity of CHS siRNA expression (Tuteja et al., 2009). Here, we investigated more tissues such as leaf, root and germinating cotyledon to ascertain the tissue specificity of CHS siRNAs in Williams. Data from multiple small RNA libraries were sequenced deeply by the Illumina high-throughput sequencing technology. The total numbers of small RNA reads were from three million to thirty million, providing sufficient data to show the tissue specificity of CHS siRNA.
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| |
|
| Overall design |
High-throughput sequencing using Genome Analyzer II and Illumina HiSeq 2000 was performed.
|
| |
|
| Contributor(s) |
Cho YB, Vodkin LO |
| Citation(s) |
24204712 |
| |
| Submission date |
Aug 09, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Lila O. Vodkin |
| E-mail(s) |
l-vodkin@illinois.edu
|
| Phone |
217-244-6147
|
| Organization name |
University of Illinois
|
| Department |
Crop Sciences
|
| Lab |
Lila Vodkin
|
| Street address |
1201 W. Gregory Dr.
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11192 |
Illumina Genome Analyzer II (Glycine max) |
| GPL15008 |
Illumina HiSeq 2000 (Glycine max) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA214792 |
| SRA |
SRP028696 |
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