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Series GSE49528 Query DataSets for GSE49528
Status Public on Aug 03, 2013
Title Comparative transcriptional analysis of Desulfovibrio alaskensis G20, a choline utilization regulator and LysX
Organism Desulfovibrio alaskensis G20
Experiment type Expression profiling by array
Summary Expression data for Desulfovibrio alaskensis strain G20 and mutants in regulator proteins grown on lactate sulfate media and then pelleted and transferred to another media when they reached stationary phase. The Choline mutant was transferred to lacte/sulfate minimal media and choline/sulfate minimal media. The LysX mutant was transferred to minimal media with lysine and rich media. G20 was transferred to minimal media, choline/sulfate minimal media, lactate/choline/sulfate minimal media, minimal media with lysine, and rich media.
We aimed to confirm or expand the regulons of each of the transposon interupted regulator mutants and compare gene expression responses of the regulators in different growth conditions.
 
Overall design 10 samples were collected: 2 regulator mutants (2 conditions each), Desulfovibrio alaskensis G20 (5 conditions), 2 replicates for G20 minimal media condition. Control sample -G20 rich media.
 
Contributor(s) Kuehl JV, Price M, Deutschbauer AM, Arkin AP, Novichkov P, Rodionov D
Citation(s) 24865553
Submission date Aug 02, 2013
Last update date Jun 03, 2014
Contact name Jennifer V Kuehl
E-mail(s) jvkuehl@lbl.gov
Phone 5106435683
Organization name Lawrence Berkeley National Laboratory
Department Physical Biosciences
Lab Arkin
Street address 1 Cyclotron Road
City Berkeley
State/province California
ZIP/Postal code 94720
Country USA
 
Platforms (1)
GPL17530 NimbleGen Desulfovibrio alaskensis str. G20 12-plex [110215_Ddesul_strG20_JK_exp]
Samples (10)
GSM1200488 G20-lactatesulfate-minimal, rep1
GSM1200489 G20-cholinesulfate-minimal
GSM1200490 G20-lactatecholinesulfate-minimal
This SubSeries is part of SuperSeries:
GSE49530 Functional genomics with a comprehensive library of transposon mutants for the sulfate-reducing bacterium Desulfovibrio alaskensis G20
Relations
BioProject PRJNA214120

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49528_RAW.tar 26.8 Mb (http)(custom) TAR (of FTR)
Raw data provided as supplementary file
Processed data included within Sample table

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