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| Status |
Public on Jun 30, 2014 |
| Title |
the analysis of piRNA shows the function of Slicer activity of AGO3 in piRNA biogenesis. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Piwi-interacting RNAs (piRNAs) silence transposons in animal germ cells. In Drosophila, the reciprocal “Ping-Pong” cycle of piRNA-directed RNA cleavage, catalyzed by the PIWI proteins Aubergine (Aub) and Argonaute3 (AGO3) through their Slicer activity, is believed to expand the population of antisense piRNAs in response to transposon expression. Whether and how the Slicer activity of AGO3/Aub promotes the process of the secondary piRNA amplification remain unclear. Here we generated transgenic flies that could express AGO3 Slicer mutant forms to ellucidate the Slicer activity of AGO3.
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| |
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| Overall design |
small-RNA libraries from 3 samples of D. mel. Ovaries.
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| Contributor(s) |
Huang H, Li Y |
| Citation(s) |
25049272 |
| Submission date |
Aug 01, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Haidong Huang |
| Organization name |
Institute of Zoology, Chinese Academy of Sciences
|
| Street address |
1 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
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| Platforms (1) |
| GPL17520 |
Ion Torrent PGM (Drosophila melanogaster) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA213903 |
| SRA |
SRP026223 |
