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Series GSE49307 Query DataSets for GSE49307
Status Public on Jul 30, 2013
Title A variant in the neuropeptide receptor npr-1 is a major determinant of Caenorhabditis elegans growth and physiology
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary The mechanistic basis for how genetic variants cause differences in phenotypic traits is often elusive. We identified a quantitative trait locus in C. elegans that affects three seemingly unrelated phenotypic traits: lifetime fecundity, adult body size, and susceptibility to the human pathogen Staphyloccus aureus. We found a QTL for all three traits arises from variation in the neuropeptide receptor gene npr-1. Moreover, we found that variation in npr-1 is also responsive for differences in 247 gene expression traits. Variation in npr-1 is known to determine whether animals disperse throughout a bacterial lawn or aggregate at the edges of the lawn. We found that the allele that leads to aggregation is associated with reduced growth and reproductive output. The altered gene expression pattern caused by this allele suggests that the aggregation behavior might cause a weak starvation state, which is known to reduce growth rate and fecundity. Importantly, we show that variation in npr-1 causes each of these phenotypic differences through behavioral avoidance of ambient oxygen concentrations. These results suggest that variation in npr-1 has broad pleiotropic effects mediated by altered exposure to bacterial food.
Overall design Two-channel experiment comparing mixed stage sample to mixed stage mixed 50:50 N2,CB4856 common reference
Contributor(s) Andersen EC, Bloom JS, Kruglyak L
Citation(s) 24586193
Submission date Jul 29, 2013
Last update date Aug 22, 2019
Contact name Leonid Kruglyak
Organization name Princeton University
Street address 110 Carl Ichan Laboratory
City Princeton
State/province NJ
ZIP/Postal code 08540
Country USA
Platforms (1)
GPL8209 Agilent-015061 C. elegans Oligo Microarray 4x44K G2519F (Probe Name version)
Samples (15)
GSM1197083 ad609 Replicate 1
GSM1197084 kyIR9 Replicate 1
GSM1197085 ky13 Replicate 1
BioProject PRJNA213610

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Supplementary file Size Download File type/resource
GSE49307_RAW.tar 199.7 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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