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Series GSE4926 Query DataSets for GSE4926
Status Public on Mar 01, 2008
Title Gene expression profiling of a mouse model of islet dysmorphogenesis
Organism Mus musculus
Experiment type Expression profiling by array
Summary In the past decade, several transcription factors critical for pancreas development have been identified. Despite this success, many of the cell surface and extracellular factors necessary for proper islet morphogenesis and function remain uncharacterized. Previous studies have shown that transgenic over-expression of the transcription factor HNF6 specifically in the pancreatic endocrine cell lineage resulted in the disruption of islet morphogenesis, including dysfunctional endocrine cell sorting, increased islet size, and failure of islets to migrate away from the ductal epithelium. We exploited the dysmorphic islets in pdx1PBHnf6 animals as a tool to identify factors important for islet morphogenesis. Genome-wide microarray analysis was used to identify differences in the gene expression profiles of late gestation and early postnatal pancreas tissue from wild type and pdx1PBHnf6 animals. We report the identification of genes with an altered expression in HNF6 Tg animals and highlight factors with potential importance in islet morphogenesis.
Keywords: disease state analysis, developmental stage analysis
Overall design Whole genome microarray analysis was used to identify differences in the gene expression profiles of late gestation and early postnatal pancreas tissue from wild type (WT) animals and HNF6 transgenic animals at a time when the events of normal islet morphogenesis are occurring (e18.5 and postnatal day 1). RNA was isolated from individual pancreata at the appropriate developmental stage. Highly pure samples were pooled according to their genotype (3-5 animals per pool) in order to obtain an adequate quantity of RNA for Affymetrix GeneChip analysis. Twelve samples were run in total, 3 replicates each of 4 groups: embryonic day 18.5, wild type mouse; embryonic day 18.5, HNF6 Transgenic mouse; post-natal day 1, wild type mouse; post-natal day 1, HNF6 Transgenic mouse.
Contributor(s) Crawford L, Tweedie E, Oh YA, Boone BE, Levy SE, Gannon M
Citation(s) 18297134
Submission date May 25, 2006
Last update date Feb 11, 2019
Contact name Laura Crawford
Organization name Vanderbilt University
Department Diabetes, Endocrinology, & Metab Division
Lab Gannon Lab
Street address 715 PRB 2220 Pierce Ave
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM111123 Pancreas_P1_WT_rep1
GSM111124 Pancreas_P1_WT_rep2
GSM111125 Pancreas_P1_WT_rep3
BioProject PRJNA95871

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Supplementary file Size Download File type/resource
GSE4926_RAW.tar 137.4 Mb (http)(custom) TAR (of CEL, CHP)
Processed data provided as supplementary file

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