|
| Status |
Public on Sep 23, 2013 |
| Title |
Gene regulation by Pax6 in the lens placode - E10.25 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Wild type or Pax6 fx/fx; Le-Cre-positive surface ectoderm from E10.25 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified and reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6 bead arrays.
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| |
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| Overall design |
Three wild type and three knockout embryos were used. Each array used the cDNA obtained from the two prospective lens tissues from one embryo.
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| |
|
| Contributor(s) |
Beebe DC, Huang J |
| Citation(s) |
21540023, 24038357 |
| Submission date |
Jul 25, 2013 |
| Last update date |
Sep 23, 2013 |
| Contact name |
David Cy Beebe |
| E-mail(s) |
beebe@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Ophthalmology and Visual Science
|
| Lab |
Campus Box 8096
|
| Street address |
660 S. Euclid Ave.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6105 |
Illumina mouse-6 v1.1 expression beadchip |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE49219 |
Histone posttranslational modifications and cell fate determination: Lens induction requires the lysine acetyltransferases CBP and p300 |
|
| Relations |
| BioProject |
PRJNA213306 |
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