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Series GSE49196 Query DataSets for GSE49196
Status Public on Sep 01, 2013
Title TALEN-Engineered AR Gene Rearrangements Reveal Endocrine Uncoupling of Androgen Receptor in Prostate Cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This experiment addressed the question of whether full length Androgen receptor and Androgen Receptor variant ARv567es have similar transciptional activities. The transcriptomes of R1-AD1, a AR full length expressing cell line, are compared in an AR on (1nM DHT) to an AR off (ethanol vehicle) state. R1-D567 was derived from R1-AD1 and modified with TALENs in such a way as to express only AR-V567es. The AR on and off states were induced with a control siRNA (AR on) or a siRNA targeting exon 1 (AR off).
 
Overall design R-AD1 was cultured for 24 hours in either base RPMI media with 1nM DHT or ethanol vehichle and RNA extracted. R1-D567 cells were electroporated with control siRNA or siRNA targeting exon 1 of AR and allowed to recover 48 hours and then cultured in base media with 1nM DHT or ethanol for 24 hrs. Each conditon was done in triplicate.
 
Contributor(s) Nyquist MD, Dehm SM
Citation(s) 24101480
Submission date Jul 25, 2013
Last update date Jan 28, 2016
Contact name Michael David Nyquist
E-mail micknyquist@gmail.com
Phone 612-961-6367
Organization name University of Minnesota
Department Genetics Cell Developmental Bio
Lab Scott Dehm and Dan Voytas
Street address Masonic Cancer Center, University of Minnesota, Mayo Mail Code 806 420 Delaware St SE
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platforms (1)
GPL10904 Illumina HumanHT-12 V4.0 expression beadchip (gene symbol)
Samples (12)
GSM1195306 R1-AD1 + DHT 1
GSM1195307 R1-AD1 + DHT 2
GSM1195308 R1-AD1 + DHT 3
Relations
BioProject PRJNA213269

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49196_non-normalized.txt.gz 3.5 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data is available on Series record
Processed data included within Sample table

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