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| Status |
Public on Nov 04, 2013 |
| Title |
Chromatin Immunoprecipitation of TRA-1 in C. elegans and C. briggsae |
| Organisms |
Caenorhabditis briggsae; Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The goal of this study is to identify and characterize sites in the C. elegans genome bound by the transcription factor TRA-1.
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| Overall design |
TRA-1 ChIP-seq was performed in the following stages of animals in duplicate: 1) L2 stage of C. elegans wild-type N2 strain; 2) L3 stage of C. elegans wild-type N2 strain; 3) young adult stage of C. elegans glp-4(bn2) mutant; 4) young adult stage of C. elegans spe-11(hc77) mutant; 5) L3 stage of C. briggsae wild-type AF16 strain. As a negative control, TRA-1 ChIP-seq was also performed in C. elegans L3 stage with tra-1(e1834) homozygous and heterozygous mutation. Input DNA was also sequenced in each condition.
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| Contributor(s) |
Berkseth M, Ikegami K, Lieb JD, Zarkower D |
| Citation(s) |
24046365 |
| Submission date |
Jul 16, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Kohta Ikegami |
| E-mail(s) |
ikgmk@uchicago.edu
|
| Phone |
(773) 834-4260
|
| Organization name |
University of Chicago
|
| Department |
Pediatrics
|
| Lab |
Ikegami Lab
|
| Street address |
900 E. 57th Street, KCBD 5240
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
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| Platforms (3) |
| GPL9269 |
Illumina Genome Analyzer II (Caenorhabditis elegans) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
| GPL16160 |
Illumina HiSeq 2000 (Caenorhabditis briggsae) |
|
| Samples (19)
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| Relations |
| BioProject |
PRJNA212403 |
| SRA |
SRP027532 |
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