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| Status |
Public on Nov 09, 2017 |
| Title |
Changes in the MicroRNA Expression Pattern Contribute to HIV-1 Tat-mediated Protection against Apoptosis and Impaired Cell Proliferation in CD4+ T Cells |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
HIV-1 infection changes the miRNA expression pattern in CD4+ T lymphocytes and its main regulator, Tat, has been appointed as an RNA silencing suppressor (RSS). Our group previously described that full-length Tat protects CD4+ T cells against FasL-mediated apoptosis and delays T-cell proliferation. Now we analyzed by microarrays and qRT-PCR the changes in the miRNA expression pattern of Jurkat cells induced by the intracellular expression of the two-exon Tat101 protein or the first-exon Tat72 in order to elucidate their role in the anti-apoptotic and anergic state exerted by Tat. It was determined that the expression of specific miRNAs such as hsa-miR-21, hsa-miR-222, hsa-miR-29a, and hsa-miR-1290 was up-regulated in Jurkat-Tat101. These miRNAs were related to the high activity of several transcription factors related to Tat such as NF-kB, SP1 and STAT3, and they repressed the expression of target mRNAs encoding proteins related to apoptosis and cell proliferation such as PTEN, PDCD4 and CDKN1B. In fact, mostly Jurkat-Tat101 showed an arrest at G2 phase and an impaired cytokinesis, resulting in giant, multilobulated nuclear cells. All these mechanisms would contribute to the role of Tat in HIV-1 immunosuppression and persistent infection, and the presence of Tat second exon would be essential.
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| Overall design |
One microgram of total RNA extracted from triplicate independent samples of each Jurkat-Tat101, Jurkat-Tat72, and control cell lines was subjected to specific labeling of miRNAs using miRCURY LNA™ microRNA Hy3/Hy5 Power labeling kit (Exiqon, Vedbaek, Denmark), according to manufacturer’s instructions.
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| Contributor(s) |
Sánchez-del Cojo M, López-Huertas M, Bermejo M, López-Campos G, Mateos E, Rodríguez-Mora S, Alcamí J, Coiras M |
| Citation(s) |
28968466 |
| Submission date |
Jul 11, 2013 |
| Last update date |
Nov 10, 2017 |
| Contact name |
Guillermo López-Campos |
| Organization name |
Queen's University of Belfast
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| Department |
Centre for Experimental Medicine
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| Street address |
97 Lisburn road
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| City |
Belfast |
| State/province |
Northern Ireland |
| ZIP/Postal code |
BT9 7BL |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17430 |
miRCURY LNA microRNA Array, 6th generation - miRBase 16.0 |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA211817 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48800_RAW.tar |
5.2 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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