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Series GSE48793 Query DataSets for GSE48793
Status Public on Nov 04, 2013
Title APC/CCCS52A2 repressed phytosulfokine signaling restricts root QC cell proliferation through ERF115 degradation
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The quiescent center (QC) plays an essential role during root development by creating a microenvironment that preserves the stem cell fate of its surrounding cells. Strikingly, in order to retain root structure, QC cells only occasionally self-renew, displaying a proliferation rate far below that of all other cells within the root meristem. Previously, the APC/CCCS52A2 ubiquitine ligase and brassinosteroid signaling pathways have been found to antagonistically control Arabidopsis thaliana QC cell proliferation. Here, we demonstrate that both pathways converge on the ERF115 transcription factor that acts as a rate-limiting factor of QC cell division through transcriptional control of the autocrine phytosulfokine PSK5 peptide hormone. ERF115 marks QC cell division but is restrained through proteolysis by the APC/CCCS52A2 ubiquitine ligase, whereas QC proliferation is driven by brassinosteroid-dependent ERF115 expression. Combined, these two antagonistic mechanisms delimit the ERF115-PSK5 activity and QC renewal. Our results reveal a unique cell cycle regulatory mechanism that accounts for the low proliferation rate of QC cells within a surrounding population of highly mitotic active cells.
 
Overall design ChIP-seq analysis of genes bound by the ERF115 transcription factor, using mock ChIP with wild type cells as negative control. Analyzed by Illumina HiSeq
 
Contributor(s) Heyman J, De Veylder L, Van Leene J, Heyndrickx K, Vandepoele K, De Jaeger G
Citation(s) 24158907
Submission date Jul 11, 2013
Last update date Sep 04, 2019
Contact name Ken Heyndrickx
E-mail(s) ken.heyndrickx@psb.vib-ugent.be
Organization name Ghent University
Department Department of Plant Biotechnology and Bioinformatics
Street address Technologiepark 927
City Gent
ZIP/Postal code 9052
Country Belgium
 
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (2)
GSM1184662 ERF115-HBH ChIP output
GSM1184663 mock PSB-d ChIP output
Relations
BioProject PRJNA211796
SRA SRP027018

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48793_RAW.tar 30.0 Kb (http)(custom) TAR (of BED, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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