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| Status |
Public on Oct 27, 2013 |
| Title |
Gene Expression Profile for knocking down Tet1 in pre-iPSCs culturing in Vc medium. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Tet1 is a hydroxylase known for its role in the conversion of 5-methylcytosines (5mC) to 5-hydroxymethylcytosines (5hmC) involved in the possible active demethylation process and gene expression regulation. As somatic cell reprogramming involves the re-activation of pluripotency genes and the silencing of somatic ones, it remains unclear the role of Tet1 in the reprogramming process. Here, we performed RNAseq for WT and knocking down Tet1 in two pre-iPSCs cell lines, pre2-2 and pre3.
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| Overall design |
Using two pre-iPSCs cell lines: pre2-2 and pre3, cultured by mES medium(+lif), transfected siCtrl/siTet1 siRNA or non-treatment, then, after 16h, the medium were replaced by mES(+lif) medium plus Vc(50ug/ml), total RNA was extracted after another 32h later for RNAseq.
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| |
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| Contributor(s) |
Pei D, Xu G |
| Citation(s) |
24162740 |
| |
| Submission date |
Jun 21, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Lihui Lin |
| E-mail(s) |
lin_lihui@gibh.ac.cn
|
| Phone |
020-32015291
|
| Organization name |
Guangzhou Institutes Of Biomedicine and Health Chinese Academy Of Science
|
| Department |
Stem cell
|
| Lab |
Pei Duanqing
|
| Street address |
No.190, Kaiyuan Street
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
GD20 |
| Country |
China |
| |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA209199 |
| SRA |
SRP026238 |
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